Abstract
Two motile, rod-shaped, Gram-stain-negative bacterial strains, TNT11T and TNT19T, were isolated from soil samples collected at Deception Island, Antarctica. According to the 16S rRNA gene sequence similarity, both strains belong to the genus Pseudomonas. Further genomic analyses based on ANI and dDDH suggested that these strains were new species. Growth of strain TNT11T is observed at 0–30 ℃ (optimum, 20 ℃), pH 4.0–9.0 (optimum, pH 6.0) and in the presence of 0–5.0% NaCl (optimum, 1% NaCl), while for TNT19T is observed at 0–30 ℃ (optimum between 15 and 20 ℃), pH 5.0–9.0 (optimum, pH 6.0) and in the presence of 0–5.0% NaCl (optimum between 0 and 1% NaCl). The fatty acid profile consists of the major compounds; C16:0 and C16:1 ω6 for TNT11T, and C16:0 and C12:0 for TNT19T. Based on the draft genome sequences, the DNA G + C content for TNT11T is 60.43 mol% and 58.60 mol% for TNT19T. Based on this polyphasic study, TNT11T and TNT19T represent two novel species of the genus Pseudomonas, for which the proposed names are Pseudomonas violetae sp. nov. and Pseudomonas emilianonis sp. nov., respectively. The type strains are Pseudomonas violetae TNT11T (= RGM 3443T = LMG 32959T) and Pseudomonas emilianonis TNT19T (= RGM 3442T = LMG 32960T). Strains TNT11T and TNT19T were deposited to CChRGM and BCCM/LMG with entry numbers RGM 3443/LMG 32959 and RGM 3442/LMG 32960, respectively.
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Data availability
The data sets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request unless otherwise stated.
Abbreviations
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
- TNT:
-
Trinitrotoluene
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Funding
This study was financially supported by Fondecyt 1200870 (J.M.P-D.), DGI-UNAB DI-03-23/INI (VC), INACH DG_13-22 (V.C.), and ANID PIA Anillo INACH ACT192057 (J.M.P-D.).
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VC: conceptualization, methodology, validation, investigation, writing—original draft, writing—review and editing, visualization. DMR: conceptualization, methodology, validation, investigation, writing—original draft, writing—review and editing, visualization. FV-I: conceptualization, methodology, validation, investigation, writing—original draft, writing—review and editing, visualization. SL-M: conceptualization, methodology, validation, investigation, writing—original draft, writing—review and editing, visualization. CD-V: methodology, validation, writing—original draft, writing—review and editing, visualization. RJM: conceptualization, investigation, resources, writing—original draft, writing—review and editing, supervision. JMP-D: conceptualization, investigation, resources, writing—original draft, writing—review and editing, supervision, project administration, funding acquisition.
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Communicated by Yusuf Akhter.
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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strains TNT11T and TNT19T are OP846032.1 and OP846033.1, respectively. The Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession NZ_JAKNRV000000000.1 (TNT11T) and NZ_JAKNRW000000000.1 (TNT19T).
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Carrasco, V., Roldán, D.M., Valenzuela-Ibaceta, F. et al. Pseudomonas violetae sp. nov. and Pseudomonas emilianonis sp. nov., two new species with the ability to degrade TNT isolated from soil samples at Deception Island, maritime Antarctica. Arch Microbiol 206, 39 (2024). https://doi.org/10.1007/s00203-023-03768-6
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DOI: https://doi.org/10.1007/s00203-023-03768-6