Abstract
During the study of microbial ecology of forest soil, two circular, white-colored bacterial colonies were isolated and labeled as strains TW38T and TW40T. Both strains were catalase positive and oxidase negative. Strains TW38T and TW40T demonstrated growth within a temperature range of 10–37 °C and 18–37 °C, respectively, and thrived within a pH range of 5.5–9.0 and 6.0–8.0, respectively. Both strains grew at 0–2.0% (w/v) NaCl concentrations. The phylogenetic analysis indicated that strains TW38T and TW40T affiliated to the genus Paenibacillus, with the closest neighbors being Paenibacillus montanisoli RA17T (98.6%) and Paenibacillus arachidis E3T (95.4%), respectively. In both strains, the sole respiratory quinone was MK-7, the signature fatty acid was antiso-C15:0, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and phosphatidylcholine. The digital DNA–DNA hybridization and the average nucleotide identity values between TW38T, TW40T, and closest reference strains were < 29.0% and < 85.0%, respectively. The DNA G+C content of TW38T and TW40T was 54.5% and 57.1%, respectively. In general, the phylogenetic, genomics, chemotaxonomic, and phenotypic data support the differentiation of TW38T and TW40T from other closest members of the genus Paenibacillus. Thus, we conclude both strains TW38T and TW40T represent novel species of the genus Paenibacillus, for which the name Paenibacillus silvisoli sp. nov. and Paenibacillus humicola sp. nov. are proposed, respectively. The type strain of Paenibacillus silvisoli is TW38T (= KCTC 43468T = NBRC 116015T) and type strain of Paenibacillus humicola is TW40T (= KCTC 43469T = NBRC 116016T).
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Data availability
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains TW38T and TW40T are OP954417 and OP954418, respectively. The whole genome shotgun sequences of strains TW38T and TW40T are CP133017 and JAQGPL000000000, respectively.
Abbreviations
- KCTC:
-
Korean Collection for Type Cultures
- KACC:
-
Korean Agricultural Culture Collection
- DSM:
-
Deutsche Sammlung von Mikroorganismen
- NBRC:
-
NITE Biological Resource Center
- ANI:
-
Average nucleotide identity
- dDDH:
-
Digital DNA–DNA hybridization
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Acknowledgements
This work was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NIBR202203112).
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This study was funded by National Institute of Biological Resources (Grant no. NIBR202203112).
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HL and DK designed the study. HL, DKC, and OBL contributed the experimental work, data analysis, and original draft preparation. HL and DKC reviewed and finalized the manuscript. DK supervised the project.
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The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains TW38T and TW40T are OP954417 and OP954418, respectively. The whole genome shotgun sequences of strains TW38T and TW40T are CP133017 and JAQGPL000000000, respectively.
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Lee, H., Chaudhary, D.K., Lim, O.B. et al. Paenibacillus silvisoli sp. nov. and Paenibacillus humicola sp. nov., isolated from forest soil. Arch Microbiol 206, 42 (2024). https://doi.org/10.1007/s00203-023-03763-x
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DOI: https://doi.org/10.1007/s00203-023-03763-x