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Plant-growth promotion by halotolerant black yeast Hortaea sp. strain PMGTC8 associated with salt crystals from solar saltern of Goa, India

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Abstract

Plant-growth-promoting microbes are sustainable alternative to improve the soil fertility and plant-growth facilitating the nutrients uptake in normal and stressed environmental conditions. Among these, halotolerant and halophilic microorganisms with plant- growth-promoting ability are better candidates that could be exploited as bioinoculants in salinity affected agriculture soils. Considering this, the current study aimed to isolate, characterize and determine the plant-growth-promoting potential of the culturable halotolerant black yeast associated with salt crystals from the solar saltern of Goa, India. The results revealed 1.3 × 104 CFU/g of viable number of colonies on 25% NaCl Tryptone Yeast Extract (NTYE) agar after 30–45 days incubation. Among ten morphologically distinct isolates, a black pigmented strain PMGTC8 was characterized as yeast-like and appeared two celled with average size of 4.30 ± 0.14 µm under scanning electron micrograph. Based on phylogenetic analysis using internal transcribed spacer (ITS) marker, the isolate showed maximum similarity to genus Hortaea. Interestingly, Hortaea sp. strain PMGTC8 (OR527117) exhibited plant-growth-promoting characteristics and caused significantly (p < 0.01) higher germination rate (93.33%), vigor index (964.22), shoot (3.95 cm) and root length (6.38 cm), and fresh weight (0.039 g) of Oryza sativa var. Korgut. This halotolerant black yeast may play a role in nutrition of the plants growing in saline soils. Conclusively, the current findings report for the first time the plant-growth-promoting potential of Hortaea sp. strain PMGTC8 associated with salt crystals of solar saltern, Goa, India for its possible use as bio-fertilizers in saline agriculture soils.

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Acknowledgements

Authors are indebted to Principal, Prof. Vikas J. Pissurlekar, P.E.S’s R.S.N. College of Arts and Science, Farmagudi, Ponda-Goa, for providing all the necessary lab facilities to carry out the research work. Author VT is thankful to Dr. Samir Damare, Principal Scientist, National Institute of Oceanography (NIO), Donapaula-Goa for giving the permission to carry out DNA extraction, purification and ITS PCR at the Biological Oceanography Department, NIO, Goa and Dr. Larissa Menezes, Assistant Professor, Microbiology, P.E.S’s R.S.N. College of Arts and Science for help rendered during phylogenetic analysis of the strain.

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  1. Sanket Gaonkar and Vaibhavi Tamse contributed equally to this work.

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  1. Department of Microbiology, P.E.S’ s R. S. N. College of Arts and Science, Farmagudi, Ponda, Goa, 403401, India

    Sanket Gaonkar, Vaibhavi Tamse, Neha Prabhu, Carolina F. E. Fernandes & Sunita Borkar

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  1. Sanket Gaonkar
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Contributions

Author VT performed the initial experiments on isolation, identification and evaluation of plant growth promoting attributes of the yeast isolate. SG proposed & designed the idea, analyzed the data and drafted the manuscript. NP performed and analyzed the plant growth promotion experiments. CF and SB helped in the results analysis and corrected the manuscript along with SG and NP.

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Correspondence to Sanket Gaonkar.

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Communicated by Yusuf Akhter.

Author SG dedicate the paper to Prof. Sarita Nazareth, Former Head, Department of Microbiology, Goa University on the occasion of her 67th Birthday.

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Gaonkar, S., Tamse, V., Prabhu, N. et al. Plant-growth promotion by halotolerant black yeast Hortaea sp. strain PMGTC8 associated with salt crystals from solar saltern of Goa, India. Arch Microbiol 206, 15 (2024). https://doi.org/10.1007/s00203-023-03740-4

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