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Transcriptomic analysis of Ralstonia solanacearum in response to antibacterial volatiles of Bacillus velezensis FZB42

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Abstract

Volatile organic compounds (VOCs), produced by a variety of microbial species and used as biological agents, have been demonstrated to play a significant role in controlling phytopathogens. In continuation of our previous studies, we aim to elucidate the underlying mechanisms and pathways involved in interactions between pathogens and microbial VOCs. In the current study, we tested how VOCs produced by Bacillus velezensis FZB42 affect the growth of Ralstonia solanacearum TBBS1 in vitro.Query The result showed that the colony growth of R. solanacearum was reduced with an inhibition rate of 0.83 ± 0.043 as compared to the control 1.7 ± 0.076, respectively. The number of viable cells of R. solanacearum was significantly decreased to 7.68 CFU/mL as compared to the control (9.02 CFU/mL). In addition, transcriptomic analysis of R. solanacearum in response to VOCs produced by FZB42 was performed to better understand the effect of VOCs on R. solanacearum. The transcriptional response of R. solanacearum to FZB42-VOCs was determined using an Illumina RNA-seq approach. The results revealed significant changes in the expression of 2094 R. solanacearum genes, including 593 upregulated and 1501 downregulated genes. To validate the RNA-seq results, the expression of 10 genes was quantified using RT-qPCR. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases were used to functionally annotate differentially expressed genes. Significant changes were observed in genes directly or indirectly related to virulence, including those related to bacterial invasion, motility, chemotaxis, and secretion systems. Overall, RNA-seq profiling provides new insights into the possible fundamental molecular mechanisms that are responsible for the reduction in growth and virulence of R. solanacearum upon application of FZB42-VOC.

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Funding

This work was supported by National Key R&D Plan Intergovernmental International Science and Technology Innovation Cooperation Project (2022YFE0121800), National Natural Science Foundation of China (31972325, 32172490), and Natural Science Foundation for Excellent Youth Scholars of Jiangsu Province, China (BK20200078).

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Authors and Affiliations

  1. Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Hafiz Abdul Samad Tahir, Qurban Ali, Abdur Rashid Khan, Qin Gu, Xuewen Gao & Huijun Wu

  2. Pakistan Tobacco Board, Ministry of National Food Security and Research, Peshawar, Pakistan

    Hafiz Abdul Samad Tahir & Waqqas Gillani

  3. Department of Plant Pathology, Faculty of Crop Protection, Sindh Agriculture University, Tandojam, 70060, Pakistan

    Faheem Uddin Rajer

  4. Department of Plant Pathology, The Islamia University of Bahawalpur, Bahawalpur, Punjab, Pakistan

    Qaisar Shakeel

  5. Muhammad Nawaz Sharif University of Agriculture, Multan, Pakistan

    Rana Binyamin

  6. Department of Plant Pathology, University of Agriculture, Faisalabad, Punjab, Pakistan

    Hafiz Muhammad Abdullah Tayyab

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  1. Hafiz Abdul Samad Tahir
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Contributions

H.W. and H.A.S.T. planned and designed this research; H.A.S.T. and Q.A performed research, methodology, and writing and editing. F.U.R., W.G., H.M.A.T., and R.B. helped with the analysis and compiled the results and data of the manuscript; F.U.R., Q.S., A.R.K., and Q.A. helped with the experiments and improved the writing. Q.G., X.G., and H.W. contributed to the critical revision of the manuscript. All the authors have read and agreed to the published version of the manuscript.

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Correspondence to Huijun Wu.

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Tahir, H.A.S., Ali, Q., Rajer, F.U. et al. Transcriptomic analysis of Ralstonia solanacearum in response to antibacterial volatiles of Bacillus velezensis FZB42. Arch Microbiol 205, 358 (2023). https://doi.org/10.1007/s00203-023-03697-4

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