Abstract
Shewanella oneidensis has demonstrated excellent potential for azo dye decolorization and degradation. However, in anaerobic environments, S. oneidensis has a narrow carbon source spectrum, which requires additional electron donors, such as sodium lactate. This increases the practical application costs for wastewater treatment. Here, we aimed to expand the carbon source utilization range of S. oneidensis FJAT-2478 by co-culturing it with Lactobacillus plantarum FJAT-7926, leveraging their commensalism relationship to develop a metabolic chain. Results showed that a 1:2 initial ratio of L. plantarum FJAT-7926 to S. oneidensis FJAT-2478 achieved a 97.16% decolorization rate of methyl orange when glucose served as the sole carbon source. This co-culture system achieved a decolorization rate comparable to that obtained using sodium lactate as an electron donor and was significantly higher than that achieved by L. plantarum FJAT-7926 (7.88%) or S. oneidensis FJAT-2478 (6.89%) alone. After undergoing five cycles, the co-culture system continued to exhibit effective decolorization. It was demonstrated that the co-culture system could use common and inexpensive carbon sources, such as starch, molasses, sucrose, and maltose, to decolorize azo dyes. For instance, 100 mg/L methyl orange could be degraded by over 98.05% within 24 h. The results indicated that the degradation rates of methyl orange were higher when L. plantarum was inoculated first, followed by a subsequent inoculation of S. oneidensis after 2 h. The co-culturing of L. plantarum FJAT-7926 and S. oneidensis FJAT-2478 proved to be an effective strategy in treating azo dye wastewater, expanding the potential practical applications of S. oneidensis.
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This work was financially supported by Fujian Special Fund for Public Interest Research (grant no: 2021R1034004).
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Yanbo Li performed the experiment, data analyses and wrote the main manuscript text; Guohong Liu helped perform the analysis with constructive discussions; Huai Shi contributed to the conception of the study. All authors reviewed the manuscript.
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Li, Y., Liu, G. & Shi, H. Expansion of carbon source utilization range of Shewanella oneidensis for efficient azo dye wastewater treatment through co-culture with Lactobacillus plantarum. Arch Microbiol 205, 297 (2023). https://doi.org/10.1007/s00203-023-03634-5
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DOI: https://doi.org/10.1007/s00203-023-03634-5