Abstract
A novel bacterium, designated as strain RS5-5T, was isolated from lake water in northwestern China. Cells of the isolate were observed to be rod shaped and Gram stain negative. Its growth occurred at 4–37 ℃, pH 6.5–9.0 and in the presence of 0–5% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain RS5-5T was most closely related to Qipengyuania sediminis GDMCC 1.2497T (97.5%), followed by Erythrobacter dokdonensis DSW-74T (97.3%) and Qipengyuania algicida GDMCC 1.2535T (97.0%). Phylogenomic analysis revealed that strain RS5-5T formed a distinct branch with the genus Parerythrobacter. The sole quinone was ubiquinone-10, and the major fatty acids (≥ 10%) were unsaturated fatty acids including C17:1 ω6c, summed feature 3 (C16:1 ω7c/C16:1 ω6c) and summed feature 8 (C18:1 ω7c/C18:1 ω6c). The polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, one unidentified sphingoglycolipid, three unidentified glycolipids, one unidentified aminoglycolipid, one unidentified aminolipid, two unidentified phospholipids and four unidentified polar lipids. Chemotaxonomic characteristics of strain RS5-5T were coincident with those of the genus Parerythrobacter members. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between strain RS5-5T and two Parerythrobacter reference strains were in the ranges of 73.2–77.7%, 69.0–78.0% and 18.9–20.4%, respectively. The genomic DNA G + C content of strain RS5-5T was 64.1%. The results of phenotypic, phylogenetic and genomic analyses suggested that strain RS5-5T represents a novel species in the genus Parerythrobacter, for which the name Parerythrobacter lacustris sp. nov. is proposed. The type strain is RS5-5T (= GDMCC 1.3163T =
KCTC 92277T).
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Data Availability
All the data generated in this study have been included in this published article and its supplementary information files. The 16S rRNA gene and the whole-genome sequences of strain RS5-5T have been deposited in the GenBank databases with the accession numbers OM267786 and JANKHH000000000 respectively.
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Acknowledgements
We are grateful to You-Ping Xu (Zhejiang University) for help in analyzing the fatty acid composition.
Funding
This work was supported by the Key R&D Program of Zhejiang (2022C03010), the Science and Technology Basic Resources Investigation Program of China (2017FY100300), the Natural Science Foundation of China (32000001), the National Science and Technology Fundamental Resources Investigation Program of China (2021FY100900) and the Open Foundation from Marine Sciences in the First-Class Subjects of Zhejiang (OFMS013).
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MW and LX designed the experiments and guided the manuscript writing. MX and HD were responsible for the major experiments, data analysis and preparation of manuscripts. J-YW and WQ assisted in enzymatic experiments and determination of fatty acids and polar lipids experiment. All authors read and approved the manuscript.
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Xamxidin, M., Dong, H., Wang, JY. et al. Parerythrobacter lacustris sp. nov., a novel member of the family Erythrobacteraceae isolated from an inland alpine lake. Arch Microbiol 205, 279 (2023). https://doi.org/10.1007/s00203-023-03616-7
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DOI: https://doi.org/10.1007/s00203-023-03616-7