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BCB1, a member of the acyl-coenzyme A synthetase family, regulates the morphogenesis and pathogenicity of Botrytis cinerea

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Abstract

Botrytis cinerea is a non-host-specific phytopathogenic fungus capable of infecting numerous cash crops. Here, we analyzed the functions of the Bcb1 gene in B. cinerea, which encodes a membrane protein belonging to the acyl-coenzyme A synthase family. Compared to the wild type, Bcb1-deletion mutants exhibited obvious morphological abnormalities, including slower vegetative growth and reduced melanin production. The absence of Bcb1 causes B. cinerea to form only small and incompletely developed infection cushions and fail to produce spores. The Bcb1 mutants displayed hypersensitivity to the membrane stressor SDS, the cell wall stressor Congo red, and the oxidative stressor H2O2 and increased resistance to intracellular osmotic stress caused by KCl compared to the wild-type strain. However, there were no differences in tolerance to extracellular osmotic stress caused by NaCl. The deletion of Bcb1 also caused a reduction in pathogenicity. The qRT‒PCR results showed that the genes Bcpks12 and Bcpks13, which are related to melanin biosynthesis, and Bcpg2, BcBOT2, and cutA, which are related to virulence, were downregulated in ∆Bcb1. These data suggest that BCB1 is important for conidial morphogenesis, and pathogenesis in B. cinerea.

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Acknowledgements

This work was supported by Grants from the National Key R&D Program of China (2019YFD1002002), the Agricultural Science and Technology Innovation Fund of Hunan Province (2022CX72), the National Key Research and Development Program of Hunan (2021NK2003), Hunan Agriculture Research System (2022-31).

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  1. Jiling Xiao and Ke Yang contributed equally to this paper.

Authors and Affiliations

  1. Hunan Provincial Key Laboratory of Pesticide Biology and Precise Use Technology, Hunan Agricultural Biotechnology Research Institute, Changsha, 410125, People’s Republic of China

    Jiling Xiao, Ke Yang & Zhihuai Liang

  2. College of Plant Protection, Hunan Agricultural University, Changsha, 410125, China

    Jiling Xiao

  3. Hunan Plant Protection Institute, Hunan Academy of Agricultural Sciences, Changsha, 410125, China

    Lin Wei

  4. Key Laboratory of Indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley, Hunan Rice Research Institute, Changsha, Hunan, China

    Yi Zhang

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  1. Jiling Xiao
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Jiling Xiao and Ke Yang wrote the main manuscript text and Zhihuai Liang performed the data analysis. Yi Zhang and Lin Wei prepared all the figures.All authors reviewed the manuscript.

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Correspondence to Zhihuai Liang.

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Xiao, J., Yang, K., Liang, Z. et al. BCB1, a member of the acyl-coenzyme A synthetase family, regulates the morphogenesis and pathogenicity of Botrytis cinerea. Arch Microbiol 205, 206 (2023). https://doi.org/10.1007/s00203-023-03540-w

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