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Biomineralization of pretilachlor by free and immobilized fungal strains isolated from paddy field

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Abstract

The excessive use of pretilachlor (a chloroacetamide herbicide) has raised concern throughout the world as it has been reported as highly toxic. The present study deals with isolating and screening pretilachlor degrading fungal strains. The strains Aspergillus ficuum (AJN2) and Aspergillus sp. (PDF1) isolated using enrichment technique were able to degrade 79% and 73% of pretilachlor respectively as analyzed using HPLC. Further, the immobilization technique was used in the study the pretilachlor degradation ability of the isolated strains. The immobilized spores of the strains AJN2 and PDF1 mineralized 84% and 95% of pretilachlor respectively. The degradation dynamics study revealed that the DT50 value of the herbicide was reduced to 2.4 d in aqueous medium due to the enhanced enzymatic activity. The enzymatic study showed high lignin peroxidase and manganese peroxidase activity by the strains AJN2 and PDF1 respectively. The study confirmed the efficient degradation of pretilachlor by Aspergillus ficuum (AJN2).

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Acknowledgements

The authors would like to express their gratitude to the management of Vellore Institute of Technology, Vellore.

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The authors declare that no funds, grants or other support were received during the preparation of the manuscript.

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Authors and Affiliations

  1. Microbial Biotechnology Laboratory, School of Bio Sciences and Technology, Vellore Institute of Technology, Vellore, Tamil Nadu, 632014, India

    Nancy Kwatra & Jayanthi Abraham

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  1. Nancy Kwatra
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  2. Jayanthi Abraham
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Contributions

JA contributed to the study conception and design. Material preparation, data collection and analysis were performed by NK. The manuscript was prepared by NK and JA.

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Correspondence to Jayanthi Abraham.

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Communicated by Yusuf Akhter.

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Kwatra, N., Abraham, J. Biomineralization of pretilachlor by free and immobilized fungal strains isolated from paddy field. Arch Microbiol 205, 188 (2023). https://doi.org/10.1007/s00203-023-03538-4

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