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Alcoholic and non-alcoholic rosé wines made with Saccharomyces cerevisiae var. boulardii probiotic yeast


The production of alcoholic and non-alcoholic rosé wines using Saccharomyces cerevisiae var. boulardii probiotic yeast is described in this study for the first time. Before and after fermentation and distillation, the volatile acidity, lactic, and malic acid levels were evaluated for S. cerevisiae var. boulardii. These contents were compared to those obtained with a standard S. cerevisiae EC-1118 yeast. We measured the levels of gluconic acid and free amino nitrogen in the musts. After fermentation and distillation, yeast viability was assessed as a function of time (0, 15 days, 3 months, and 6 months), both at ambient temperature (25 ± 0.5 °C) and refrigerator temperature (4 ± 0.5 °C). The outcomes revealed that the rosé wine made with S. cerevisiae var. boulardii had the same values and preliminary sensory characteristics as other commercial wines made with S. cerevisiae EC-1118. The S. cerevisiae var. boulardii yeast successfully survived the high alcohol level produced during fermentation and vacuum distillation. The study also revealed that this unique rosé wine retains its probiotic viability for at least 6 months when stored at room temperature or in the refrigerator, making it a suitable candidate for large-scale production where long storage intervals are required by both producers and consumers.

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All data generated or analyzed during this study are included in this published article.


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This research was funded by the Fundación Universidad Católica de Valencia San Vicente Mártir by means of Grant Nº 2020-231-006UCV.

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Conceptualization: JM-C and ÁS-A conceived the idea of this work. Experiments: JM-C, AT-M, AC-V, LP-C, MM; data curation: AT-M, ÁS-A, and JM-C; formal analysis: ÁS-A; supervision: ÁS-A, JM-C, and MM; investigation: AT-M, C-V, M, JM-C, and ÁS-A; writing—original draft: JM-C and ÁS-A; writing—reviewing and editing: AT-M, AC-V, MM, JM-C, and ÁS-A; funding acquisition: ÁS-A.

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Correspondence to Ángel Serrano-Aroca.

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The authors declare no competing interests.

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Communicated by Yusuf Akhter.

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Mulero-Cerezo, J., Tuñón-Molina, A., Cano-Vicent, A. et al. Alcoholic and non-alcoholic rosé wines made with Saccharomyces cerevisiae var. boulardii probiotic yeast. Arch Microbiol 205, 201 (2023).

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