Abstract
A Gram-staining-negative, non-motile, rod-shaped or curved rod-shaped, moderately halophilic bacterium, designated C176T, was isolated from Yuncheng Salt Lake in Shanxi Province, P.R. China. The optimal temperature, salinity and pH for growth of strain C176T was 37 °C, 6% (w/v) NaCl and 7.5. Phylogenetic analysis using 16S rRNA gene sequences indicated strain C176T has the highest similarity with Spiribacter salinus LMG 27464 T (97.7%), following by the S. halobius E85T (97.6%), S. curvatus DSM 28542 T (97.2%), S. roseus CECT 9117 T (97.0%) and S. vilamensis DSM 21056 T (96.9%). The ANI and dDDH values between strain C176T and S. salinus LMG 27464 T were 69.8 and 17.7%, respectively. The DNA G + C content of genome for strain C176T was 54.1%. Summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) and C16:0 were detected as its major fatty acids, with content of 38.7 and 28.6% respectively, while Q-8 was the predominant ubiquinone. The major polar lipids of strain C176T contained phospholipid, phosphatidylglycerol and phosphoglycolipid. In accordance with results of polyphasic taxonomy, strain C176T is considered as a novel species of the genus Spiribacter, for which the name Spiribacter salilacus sp. nov. is proposed. The type strain is C176T (= MCCC 1H00417T = KCTC 72692 T).
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Data availability
The data presented in the study are deposited in the GenBank. The accession number for 16S rRNA gene sequence and the draft whole-genome data of strain C176T are MT804405 and SAMN13193768, respectively.
Abbreviations
- ANI:
-
Average nucleotide identity
- AAI:
-
Amino acid identity
- dDDH:
-
Digital DNA-DNA hybridization
- MA:
-
Marine agar 2216
- MB:
-
Marine broth 2216
- GGDC:
-
Genome-to-genome distance calculator
- MEGA:
-
Molecular evolutionary genetics analysis
- HPLC:
-
High-performance liquid chromatography
- TLC:
-
Thin-layer chromatography
- FAME:
-
Fatty acids methyl esters
- BGI:
-
The Beijing Genomics Institute
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Acknowledgements
The implementation of scanning electron microscope was supported by the Physical-Chemical Materials Analytical and Testing Center of Shandong University at Weihai
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This work was supported by Science & Technology Fundamental Resources Investigation Program (Grant No. 2022FY101100).
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All authors contributed to the study conception and design. Material preparation and data collection were performed by TTZ, XYZ and JCW. The data analysis was performed by TTZ and DL. The first draft of the manuscript was written by TTZ, DL and JCW. ZJD has reviewed and given guidance to this project. All authors commented on previous versions of the manuscript. All authors have read and approved the final manuscript.
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Zhang, TT., Liu, D., Zhang, XY. et al. Spiribacter salilacus sp. nov., a novel moderately halophilic bacterium isolated from a saline lake in China. Arch Microbiol 205, 166 (2023). https://doi.org/10.1007/s00203-023-03511-1
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DOI: https://doi.org/10.1007/s00203-023-03511-1