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Paraprobiotics strains accelerate wound repair by stimulating re-epithelialization of NIH-3T3 cells, decreasing inflammatory response and oxidative stress

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Abstract

The present study aimed to evaluate the potential and specificity of the inflammatory and antioxidant response of Microbe‐Associated Molecular Patterns (MAMPs) in NIH-3T3 fibroblast cells, as well as in the healing process of skin wounds. Cells (NIH-3T3) were cultivated in supplemented specific medium. NIH-3T3 cells were treated with MAMPs (Bifidobacterium lactis or Lactobacillus casei or Lactobacillus gasseri or Lactobacillus paracasei or Streptococcus thermophilus), at two concentrations and insulted with LPS or H2O2. Cell viability, myeloperoxidase activity, nitrite/nitrate, oxidative damage and inflammatory parameters were measured. In addition, scratch assay was performed. Significant scratch closure was observed after 24 h and 48 h, and the effect of 0.1 g/mL MAMPs on wound healing was found to be highly statistically significant. In the viability cellular assay, Lactobacillus showed better response in 0.1 g/mL dose, whereas B. lactis and S. thermophilus showed better response in 0.01 g/mL dose. There was reduction in IL-6 and IL-1β levels in all treatments insulted with LPS. MAMP’s showed preventive efficacy in reducing the effects caused by LPS. The MAMP’s action in decreasing the production of ROS, inflammatory activity and increasing cell viability, besides significant cell proliferation during wound healing processes suggests remodeling mechanisms and new possibilities for wound healing.

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Data availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

UNESC, Biohall Research and Innovation; Gabbia Biotechnology.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico, MONIQUE MICHELS-PDI.

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Authors and Affiliations

  1. Gabbia Biotechnology, Barra Velha, Santa Catarina, Brazil

    Monique Michels, Ana Paula Lorenzen Voytena, Marina Rossetto, Fernanda Ramlov & Gabriel Fernandes Alves Jesus

  2. Biohall Research and Innovation, Itajaí, Santa Catarina, Brazil

    Monique Michels & Gabriel Fernandes Alves Jesus

  3. Laboratory of Experimental Pathophysiology-Graduate Program in Health Sciences, University of Southern Santa Catarina (UNESC), Criciúma, Brazil

    Emily Córneo, Luana Bezerra Gonçalves Rocha, Rodrigo Dias & Felipe Dal-Pizzol

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  1. Monique Michels
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Contributions

MM, GFAJ—conceptualization and design of the study; Data curation; Formal analysis and Methodology; Roles/Writing—original draft. EC, LBGR, RD, APLV, MPR, FR—conceptualization and design of the study; Methodology FDP—conceptualization and design of the study; Project administration; Supervision; Funding acquisition; Writing—review & editing. All authors approved final version submitted. We confirm that the manuscript has been read and approved by all named authors and that there are no other persons who satisfied the criteria for authorship but are not listed. We further confirm that the order of authors listed in the manuscript has been approved by all of us.

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Correspondence to Monique Michels.

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Conflict of interest

Gabbia Biotechnology are developing paraprobiotics for the commercial purposes. Marina Rosseto, Ana Paula Voytena and Fernanda Ramlov are members of Gabbia Biotechnology. Monique Michels and Gabriel Alves Jesus are members of Biohall Research and Innovation. The authors declared no potential conflicts of interest.

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Communicated by Erko Stackebrandt.

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203_2023_3469_MOESM1_ESM.png

Supplementary file1 NIH-3T3 cells were insulted with LPS or H2O2 that increase ROS generation and cytokines production. After treatment with MAMP’s (paraprobiotics), these parameters decreased. Besides this, re-epithelization occurs after treatment (PNG 625 KB)

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Michels, M., Córneo, E., Rocha, L.B.G. et al. Paraprobiotics strains accelerate wound repair by stimulating re-epithelialization of NIH-3T3 cells, decreasing inflammatory response and oxidative stress. Arch Microbiol 205, 134 (2023). https://doi.org/10.1007/s00203-023-03469-0

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