Abstract
Antibiotic resistance poses a significant threat to the global health, food security, and environment. In poultry and livestock, antibiotics are beneficial since they improve poultry performance and are economically effective. Therefore, it is crucial to search for alternatives that can be environmentally safe and successful in treating these infections. In this study, we employed molecular docking to evaluate lemon peel phytochemical’s protein binding capability against various poultry pathogens. The nanoparticles (LP AgNPs) obtained from the lemon peel were characterized and tested for their antibacterial activity against more poultry pathogens. LP AgNPs were characterized by using UV–Visible absorption spectra, which revealed an absorption peak at a wavelength of 420–440 nm. The FT–IR analysis demonstrated that flavonoids and phenolic acids acted as capping, reducing, and stabilizing agents during the biosynthesis of AgNPs. EDAX showed a strong peak was observed at 3 keV which revealed the absorption of metallic silver nanoparticles. The mean diameter was from 2 to 20 nm through HRTEM. Zeta potential of the LP AgNps at − 17.2 mV showed the high stability of the green synthesized AgNps. Maximum inhibitory concentrations of LP AgNps against the isolated poultry pathogens were 50 μg/ml concentration. The toxicity tests were performed in the Vigna radiata seedlings and Artemia nauplii, which showed less toxic effects and eco-friendly nature of the LP AgNps. LP AgNps have the potential to treat antibiotic resistant poultry pathogens, thereby paving the way for the development of value-added novel products incorporated with nanoparticles for treating various infection caused by antibiotic-resistant poultry pathogens.
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Acknowledgements
The authors are thankful to B.S. Abdur Rahman Institute of Science and Technology, Chennai for providing research facilities in school of life sciences. The authors also gratefully acknowledge the Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi. (Ref No. DST/WOS-B/2018/1583-HFN (G)).
Funding
Ministry of Science and Technology, Department of Science and Technology (KIRAN Division) (GoI), New Delhi. (Ref No. DST/WOS-B/2018/1583-HFN (G)).
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Srividhyaa, K., Ranjani, S. & Hemalatha, S. Citrus limon phytocompounds decorated nanoparticles control poultry pathogens. Arch Microbiol 205, 124 (2023). https://doi.org/10.1007/s00203-023-03462-7
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DOI: https://doi.org/10.1007/s00203-023-03462-7