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Hominibacterium faecale gen. nov., sp. nov., an anaerobic l-arginine-degrading bacterium isolated from human feces

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Abstract

Two anaerobic, mesophilic bacteria SF3T and ASD5510 were isolated from human feces in two different countries. Strain SF3T shared 99.9% of 16S rRNA gene sequence similarity with strain ASD5510, and 92.8% similarity with the most similar strain Aminipila butyrica DSM 103574T. Strain SF3T was an anaerobic, Gram-stain-positive bacterium. Cells of strain SF3T were short rods with 0.3–0.4 µm in width × 2.0–2.4 µm in length and occurred mostly in pairs or short chains. Spore formation was not observed. The strain grew optimally at 35 °C (range from 20 to 45 °C), pH 7.5 (pH 6.0–8.5) and without NaCl addition (range from 0 to 20 g l−1 NaCl). Yeast extract was an essential growth factor for strain SF3T, l-arginine and γ-aminobutyrate were utilized as substrates for growth. The major cellular fatty acids were iso-C15:0 and C16:0 DMA. The main polar lipids were aminophospholipid (APL), diphosphatidylglycerol (DPG) and phosphatidylethanolamine (PE). The G + C content of the genomic DNA of the strain SF3T was 47.38 mol %. The paired genomic average amino acid identity (AAI) and percentage of conserved proteins (POCP) values showed relatedness of less than 61.0 and 39.4% with type strains of order Eubacteriales. On the basis of phenotypic, phylogenetic and phylogenomic evidence strain SF3T constitutes a novel species in a novel genus, for which the name Hominibacterium faecale gen. nov., sp. nov. is proposed. The type strain is SF3T (= CCAM 730T = JCM 34755T = KCTC 25324T).

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Data Availability

The authors confirm that the data supporting the findings of this study are available within the article and its supplementary materials. 16S rRNA gene sequence and draft genome are available in National Center for Biotechnology Information.

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Acknowledgements

We thank Professor Paul A Lawson at Oklahoma university for checking the etymology of the strain name.

Funding

This study was supported by the Agricultural Science and Technology Innovation Project of the Chinese Academy of Agriculture Science (CAASASTIP-2016-BIOMA), Central Public-interest Scientific Institution Basal Research Fund (1610012017002_05103, Y2021PT02), and Chengdu Major Scientific and Technological Program (2021-YF08-00030-GX).

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Contributions

NB, performed the experiment, data analysis and wrote the manuscript; MY helped perform the experiment and data analysis; AVC isolated strain ASD5510 and genome sequencing; JL performed the phylogenetic analysis; QW helped isolate the culture; LD helped incubate the culture; KW helped perform the analysis with constructive discussions; SM contributed to the manuscript revision; BAE contributed manuscript preparation and edition; LC contributed to the conception of the study, funding acquisition, supervision and manuscript review.

Corresponding author

Correspondence to Lei Cheng.

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The authors declare that there are no conflicts of interest.

Additional information

Communicated by Erko Stackebrandt.

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The GenBank accession numbers of the 16S rRNA gene and draft-genome sequences for strain SF3T are MZ297465 and JAJAGH000000000, for strain ASD5510 are MT908947.1 and JAOSHN000000000.

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Supplementary file1 (DOCX 2912 KB)

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Borhanudin, N., Yang, M., Chaplin, A.V. et al. Hominibacterium faecale gen. nov., sp. nov., an anaerobic l-arginine-degrading bacterium isolated from human feces. Arch Microbiol 205, 33 (2023). https://doi.org/10.1007/s00203-022-03365-z

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  • DOI: https://doi.org/10.1007/s00203-022-03365-z

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