Abstract
Bovine mastitis is an important disease in dairy cows, and Staphylococcus aureus is the most prevalent microorganism. Bacteriophages are considered an alternative to treat bacterial infections due to antimicrobial resistance crisis. In this study, we isolated and characterized novel S. aureus temperate phages, namely B_UFSM4 and B_UFSM5, from bovine milk. The complete genomes of B_UFSM4 and B_UFSM5 have 41.396 bp and 41.829 bp, respectively. The viruses have double-stranded DNA and linear architecture. Phylogenic similarity was observed by proteome with Staphylococcus phage phiPV83, CN125 and JS01. Therefore, the phages were classified into the family Siphoviridae, genus Biseptimavirus and order Caudovirales. In the host range, the B_UFSM4 and B_UFSM5 had lytic activity of 45.8% and 54.16%, respectively, inclusive on isolates from Staphylococcus sciuri and Rothia terrae. Thus, in this study, species novel of S. aureus temperate phages was isolated and characterized, these phages reveal similarities to each other; however, they are distinct from other species of S. aureus phages of the family Siphoviridae.
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Data availability
Nucleotide sequence data reported are available in the GenBank database under the accession number MW147366 for phage B_UFSM4 and MW192778 for B_UFSM5.
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Acknowledgements
The authors acknowledge the support received from: Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) (financial code 001); Setor de Virologia at Universidade Federal de Santa Maria (SV/UFSM); Centro de Microscopia da Zona Sul—FURG (CEME-SUL) at Universidade Federal de Rio Grande (FURG); and Prof Dr. Daniel Mendes Pereira Ardisson de Araújo of Departamento de Bioquimica e Biologia Molecular at UFSM that contributed to the development of this research. This research was also partially supported by Financiadora de Estudos e Projetos (FINEP, grant number 0112.0113).
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Conceptualization: BMB; SAB. Methodology: BMB; ESP; DIBP; JFC; SAB. Formal analysis and investigation: BMB; GFG; DIBP; SC; APMV; FQM; SAB. Writing—original draft preparation: BMB; SAB. Writing—review and editing: BMB; ESP; DIBP; SC; APMV; FQM; GFG; LAS; JFC; SAB. Funding acquisition: SAB. Resources: FQM; JFC; SAB. Supervision: SAB.
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Communicated by Erko Stackebrandt.
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Barasuol, B.M., Cargnelutti, J.F., Sangioni, L.A. et al. Characterization of novel of temperate phages of Staphylococcus aureus isolated from bovine milk. Arch Microbiol 204, 680 (2022). https://doi.org/10.1007/s00203-022-03296-9
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DOI: https://doi.org/10.1007/s00203-022-03296-9