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Leuconostoc mesenteroides utilizes glucose fermentation to produce electricity and ameliorates high-fat diet-induced abdominal fat mass

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Abstract

Bacteria capable of producing electricity in intestinal microbiota have been discovered. However, no studies have explored butyric acid which generated by electrogenic bacteria on the host organism have significant physiological impacts on certain organs. We found that the capacity for electrical current generation by the commensal gut Leuconostoc mesenteroides EH-1 (L. mesenteroides EH-1) during glucose fermentation. The electricity production was essential for the gut colonization of L. mesenteroides EH-1 since the inhibition of electricity production by cyclophilin A inhibitor (TMN355) significantly diminished the number of bacteria attached to the human gut epithelial cell surface. The adipocyte differentiation contributes to the increased 4-hydroxy-2-nonenal (4-HNE), considered as a biomarker of reactive oxygen species (ROS). The effect of intestinal electrogenic microbiota in the high-fat diet (HFD)-induced 4-HNE and abdominal fat accumulation in mice was investigated in this study. The oral administration of glucose with a butyric acid-producing L. mesenteroides EH-1 bacterium attenuated the expression of 4-HNE and abdominal fat. The level of 4-HNE and abdominal fat depot were markedly increased in mice administered with cyclophilin A inhibitor-pretreated bacteria or GLPG-0974, an antagonist of free fatty acid receptor 2 (Ffar2). Our studies suggest a novel means by which the probiotic bacteria can modulate fat mass deposition and oxidative stress via the cyclophilin A-mediated electron production and the butyric acid-activated Ffar2 pathway.

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Acknowledgements

This work was supported by Ton Duc Thang University

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  1. Faculty of Applied Sciences, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Minh Tan Pham & Thi Dung Tran

  2. Department of Biomedical Sciences and Engineering, National Central University, Taoyuan, Taiwan

    Enkhbat Zayabaatar

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  1. Minh Tan Pham
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  2. Thi Dung Tran
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MTP was in charge of experiments, wrote, edited, and reviewed manuscript; TDT designed and interpreted study; EZ analyzed data. All authors approved the final version of the manuscript. MTP is the guarantor of this work.

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Pham, M.T., Tran, T.D. & Zayabaatar, E. Leuconostoc mesenteroides utilizes glucose fermentation to produce electricity and ameliorates high-fat diet-induced abdominal fat mass. Arch Microbiol 204, 670 (2022). https://doi.org/10.1007/s00203-022-03281-2

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