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Investigation of microplastic accumulation in Rastrelliger kanagurta fish gut and microplastic degradation behaviour of existing gut bacteria Pseudomonas sp.

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Abstract

Microplastic is a minute particle of chemical pollutant in marine environment and classified as less than 5 mm size. The microplastics could not degrade for long years and they are ingested, incorporated, and accumulated in tissues of living organisms, particularly can cause various ecotoxicological effects for their behavioural change, cytotoxicity, neuro-toxicity effects, liver stress, etc. This preliminary study was investigated the abundance and accumulation of microplastic in marine fish of Indian mackerel (Rastrelliger kanagurta) gut region. Further, we identified the microplastic through stereomicroscope in Indian mackerel fish size up to 0.02 mm. In FT-IR analysis were identified the chemical group which were represents as nylon. In GC–MS analysis were identified that hexa decanoic acid and methyl ester plastic compounds as well as identify and screened the microplastic degrading bacteria from fish gut through partial 16S rRNA gene sequencing analysis it was shows that the isolate reveals a Pseudomonas sp. As a result, it is possible that gut bacteria have a probiotic role in fish gut to may degrade microplastics.

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Acknowledgements

We are thankful to Bharathidasan University Research fellowship (URF), Trichy, India for their financial support in the form of RUSA 2.0 (Biological Science) and TANSCHE to carry out this work.

Funding

The first author thanks the Research Foundation of Bharathidasan University for providing the University Research Fellowship (URF) (021505/ URF/DIR-RES/2019 Date: 22.10.2019).

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Authors and Affiliations

  1. Rhizosphere Biology Laboratory, Department of Microbiology, Bharathidasan University, Tiruchirappalli, Tamil Nadu, 620024, India

    Kayalvizhi Rajendran, Ramya Rajendiran, Ragaranjani Ravichandran & Rajesh Kannan Velu

Authors
  1. Kayalvizhi Rajendran
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  2. Ramya Rajendiran
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  3. Ragaranjani Ravichandran
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  4. Rajesh Kannan Velu
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Contributions

VRK—Experimental Design, Conceptualization, Manuscript Revision, Supervision; Validation; Visualization. RK—Data curation; Formal analysis; Project administration; Resources; Software; Supervision; Validation; Visualization; Roles/Writing – original draft. R. Ramya—Writing—review and editing. R. Ragaranjani—Investigation; Methodology. All authors conceived and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Rajesh Kannan Velu.

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The authors declare no conflict of interest.

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Communicated by Erko Stackebrandt.

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Rajendran, K., Rajendiran, R., Ravichandran, R. et al. Investigation of microplastic accumulation in Rastrelliger kanagurta fish gut and microplastic degradation behaviour of existing gut bacteria Pseudomonas sp.. Arch Microbiol 204, 626 (2022). https://doi.org/10.1007/s00203-022-03241-w

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  • DOI: https://doi.org/10.1007/s00203-022-03241-w

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