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Paenibacillus mellifer sp. nov., isolated from gut of the honey bee Apis mellifera

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Abstract

An isolate, designated MBLB2552T, was isolated from the gut of the honey bees (Apis mellifera L.) and identified as a member of the genus Paenibacillus based on the sequences of the 16S rRNA gene sequences. The most closely related species to strain MBLB2552T were Paenibacillus timonensis 2301032 T, Paenibacillus barengoltzii NBRC 101215 T, and Paenibacillus macerans IAM 12467 T, with similarity values of 98.1, 97.21 and 97.0%, respectively, based on 16S rRNA gene sequences. The genome size and G + C content of MBLB2552T were 5.2 Mb and 52.4%, respectively. The Ortho average nucleotide identity (OrthoANI) and in silico DNA–DNA hybridization (isDDH) values between strain MBLB2552T and the type strains of the closest species were below the species delineation threshold. Comparative genomic analysis showed that most core POGs of strain MBLB2552T and other related taxa were related to translation, ribosomal structure and biogenesis (J) and carbohydrate metabolism in the COG category and KEGG pathways, respectively. Strain MBLB2552T was Gram stain-positive, spore-forming, rod-shaped, facultative anaerobic, motile, and grew at 20‒45 °C in 0‒2% (w/v) NaCl at pH 6.0‒9.0. The major polar lipids identified were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylserine, unidentified polar lipids, and an unidentified glycolipid. We propose that strain MBLB2552T represents the type strain of the genus Paenibacillus and its name Paenibacillus mellifer sp. nov. is proposed. The type of strain was MBLB2552T (= JCM 35371 T = KCTC 43386 T).

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Funding

This work was carried out with the support of Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ015755022022). This work was also supported by Research Assistance Program (2021) in the Incheon National University.

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Author notes

  1. Eui-Sang Cho and Chi Young Hwang have contributed equally to this work.

Authors and Affiliations

  1. Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Eui-Sang Cho, Chi Young Hwang & Myung-Ji Seo

  2. Department of Life Sciences, Incheon National University, Incheon, 22012, Republic of Korea

    Hyung Wook Kwon

  3. Convergence Research Center for Insect Vectors, Incheon National University, Incheon, 22012, Republic of Korea

    Hyung Wook Kwon

  4. Division of Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

  5. Research Center for Bio Materials and Process Development, Incheon National University, Incheon, 22012, Republic of Korea

    Myung-Ji Seo

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  1. Eui-Sang Cho
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ESC and CYH performed all the experiments, analyzed the data, and drafted the manuscript. HWK acquired funding. MJS designed all the experiments and supervised the manuscript.

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Correspondence to Myung-Ji Seo.

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Cho, ES., Hwang, C.Y., Kwon, H.W. et al. Paenibacillus mellifer sp. nov., isolated from gut of the honey bee Apis mellifera. Arch Microbiol 204, 558 (2022). https://doi.org/10.1007/s00203-022-03178-0

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