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Microbulbifer okhotskensis sp. nov., isolated from a deep bottom sediment of the Okhotsk Sea

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Abstract

A Gram-negative, aerobic, non-motile bacterium КMM 9862T was isolated from a deep bottom sediment sample obtained from the Okhotsk Sea, Russia. Based on the 16S rRNA gene and whole genome sequences analyses the novel strain КMM 9862T fell into the genus Microbulbifer (class Gammaproteobacteria) sharing the highest 16S rRNA gene sequence similarities of 97.4% to Microbulbifer echini AM134T and Microbulbifer epialgicus F-104T, 97.3% to Microbulbifer pacificus SPO729T, 97.1% to Microbulbifer variabilis ATCC 700307T, and similarity values of < 97.1% to other recognized Microbulbifer species. The average nucleotide identity and digital DNA–DNA hybridization values between strain КMM 9862T and M. variabilis ATCC 700307T and M. thermotolerans DSM 19189T were 80.34 and 77.72%, and 20.2 and 19.0%, respectively. Strain КMM 9862T contained Q-8 as the predominant ubiquinone and C16:0, C16:1 ω7c, C12:0, and C10:0 3-OH as the major fatty acids. The polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylcholine, an unidentified aminophospholipid, an unidentified aminolipid, two unidentified phospholipids, phosphatidic acid, and an unidentified lipid. The DNA G+C content of 49.8% was calculated from the genome sequence. On the basis of the phylogenetic evidence and distinctive phenotypic characteristics, the marine bacterium KMM 9862T is proposed to be classified as a novel species Microbulbifer okhotskensis sp. nov. The type strain of the species is strain KMM 9862T (= KACC 22804T).

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Acknowledgements

We would like to thank Dr. Soon-Wo Kwon, the Korean Agricultural Culture Collection (KACC), Agricultural Microbiology Division, National Academy of Agricultural Science, Suwon, Korea, for providing the type strain Microbulbifer echini KACC 18258T for comparative analyses. The DDBJ/ENA/GenBank accession numbers for the 16S rRNA gene and genome sequences of strain KNN 9862T are OL677189 and JALBWM000000000, respectively. The version described in this paper is version JALBWM000000000.1.

Funding

This research work was supported by Grant of the Ministry of Science and Higher Education, Russian Federation 13.1902.21.0012 (contract No 075-15-2020-796).

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Authors and Affiliations

  1. G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku 159, Vladivostok, 690022, Russia

    Lyudmila Romanenko, Valeriya Kurilenko, Nadezhda Otstavnykh, Marina Isaeva & Valery Mikhailov

  2. A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Palchevskogo str. 17, Vladivostok, 690041, Russia

    Peter Velansky

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  1. Lyudmila Romanenko
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Contributions

L.R. and V.K. designed the study, isolated and tested bacteria, and wrote the first draft of the manuscript. N.O. and M.I. performed sequencing and phylogenetic analyses; P.V. did lipid analyses, M.I. and V.M. managed the study and analyzed the results. All authors edited and agreed to the published version of the manuscript.

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Correspondence to Lyudmila Romanenko.

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Communicated by Erko Stackebrandt.

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Romanenko, L., Kurilenko, V., Otstavnykh, N. et al. Microbulbifer okhotskensis sp. nov., isolated from a deep bottom sediment of the Okhotsk Sea. Arch Microbiol 204, 548 (2022). https://doi.org/10.1007/s00203-022-03161-9

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