Abstract
In the present study, the taxonomic position of Bacillus tepidiphilus was re-evaluated. Bacillus tepidiphilus (B. tepidiphilus) SYSU G01002T showed the highest 16S rRNA gene sequence similarity with the type strain Peribacillus alkalitolerans (P. alkalitolerans) (97.7%). In the phylogenetic (based on 16S rRNA sequence) and phylogenomic (based on 71 bacterial single-copy genes) trees, B. tepidiphilus SYSU G01002T clade with the members of the genus Peribacillus. The amino acid identity (AAI) value of B. tepidiphilus SYSU G01002T was highest with P. alkalitolerans KCTC 33631T (73.6%). The AAI value between B. tepidiphilus SYSU G01002T and P. alkalitolerans KCTC 33631T was above the cutoff level for genus delineation. The average nucleotide identity (ANI) between B. tepidiphilus and P. alkalitolerans KCTC 33631T was 74.1%, which was below the ANI value (95–96%) for species delineation. Based on the phylogenetic, phylogenomic, AAI, and ANI analysis, Bacillus tepidiphilus is proposed to transfer to the genus Peribacillus as Peribacillus tepidiphilus comb. nov. The type strain is SYSU G01002T (= KCTC 43131T = CGMCC 1.17491T).
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Funding
This work was financially supported by the Office of the Ministry of Higher Education, Science, Research and Innovation; and the Thailand Science Research and Innovation through the Kasetsart University Reinventing University Program 2022. MPNR has been awarded a post-doctorate fellowship under KU Reinventing University Program 2022.
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MPNR designed the study and conducted the genome analysis. AD and AB revised and corrected the draft. MPNR and AT supervised the study and revised the final manuscript.
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Narsing Rao, M.P., Dhulappa, A., Banerjee, A. et al. Transfer of Bacillus tepidiphilus Narsing Rao et al. 2021 to the genus Peribacillus as Peribacillus tepidiphilus comb. nov.. Arch Microbiol 204, 545 (2022). https://doi.org/10.1007/s00203-022-03160-w
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DOI: https://doi.org/10.1007/s00203-022-03160-w