Skip to main content

Advertisement

Log in

Cationic polymer contributes to broaden the spectrum of vancomycin activity achieving eradication of Pseudomonas aeruginosa

  • Original Paper
  • Published:
Archives of Microbiology Aims and scope Submit manuscript

Abstract

Vancomycin (VAN) is unable to penetrate the outer membrane of Gram-negative bacteria and reach the target site. One approach to overcome this limitation is to associate it with compounds with permeabilizing or antimicrobial properties. Eudragit E100® (Eu) is a cationic polymer insufficiently characterized for its potential antimicrobial action. Eu-VAN combinations were characterized, the antimicrobial efficacy against Pseudomonas aeruginosa was evaluated and previous studies on the effects of Eu on bacterial envelopes were extended. Time-kill assays showed eradication of P. aeruginosa within 3–6 h exposure to Eu-VAN, whilst VAN was ineffective. Eu showed regrowth in 24 h and delayed colony pigmentation. Although permeabilization of bacterial envelopes or morphological alterations observed by TEM and flow cytometry after exposure to Eu were insufficient to cause bacterial death, they allowed access of VAN to the target site, since Eu-VAN/Van-FL-treated cultures showed fluorescent staining in all bacterial cells, indicating Van-FL internalization. Consequently, Eu potentiated the activity of an otherwise inactive antibiotic against P. aeruginosa. Moreover, Eu-VAN combinations exhibited improved physicochemical properties and could be used in the development of therapeutic alternatives in the treatment of bacterial keratitis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

Data availability

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

Download references

Acknowledgements

MC, LC and VR would like to thank CONICET- Argentina for scholarships. The authors especially thank Dr. Paul Hobson, native speaker, for revising the manuscript. They also thank Dr. Santiago Palma for providing useful suggestions.

Funding

This work was supported by grants from Secretaria de Ciencia y Tecnología- Universidad Nacional de Córdoba (Res No 411/18); and FONCyT-Agencia Nacional de Promoción de la Investigación, Desarrollo Tecnológico e Innovación (PICT2012-0173).

Author information

Authors and Affiliations

Authors

Contributions

MC, LC and VR designed the protocols, carried out the experimental part and analyzed data. SG contributed in TEM image acquisition and analysis. MC drafted the manuscript with FA. FA conceived the study, acquired funding and resources, supervised the design of experiments and data interpretation, and completed the writing of the manuscript.

Corresponding author

Correspondence to Fabiana L. Alovero.

Ethics declarations

Conflict of interest

The authors have no relevant financial or non-financial interests to disclose.

Ethical approval

Non-applicable.

Consent to participate

Non-applicable.

Consent for publication

Non-applicable.

Additional information

Communicated by Erko Stackebrandt.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Corti, M.B., Campagno, L.P., Romero, V.L. et al. Cationic polymer contributes to broaden the spectrum of vancomycin activity achieving eradication of Pseudomonas aeruginosa. Arch Microbiol 204, 507 (2022). https://doi.org/10.1007/s00203-022-03117-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s00203-022-03117-z

Keywords

Navigation