Abstract
Rumex gmelinii Turcz. (RGT) is a medicinal plant of the genus Rumex, family Polygonaceae. Our research group isolated an endophytic fungus, Plectosphaerella cucumerina (Strain J-G) from RGT, which could significantly promote host growth when co-cultured with host seedlings. In this study, we used transcriptome analysis and verification experiments to explore the molecular mechanisms underlying this growth-promoting effect. We found that, during co-culture with Strain J-G, the expression of genes encoding key enzymes in amino acid metabolism and carbohydrate synthesis and metabolism were up-regulated in RGT tissue culture seedlings, providing additional substrate and energy for plant growth. In addition, the expression of genes encoding the responser of RGT seedlings to hormones, including auxin and cytokinin, were significantly enhanced, promoting plant growth and development. Furthermore, RGT seedling defense systems were mobilized by Strain J-G; therefore, more secondary metabolites and substances involved in stress resistance were produced, ensuring normal plant growth and metabolism. The research showed Strain J-G significantly promote the accumulation of biomass and effective components of RGT, which provide basis for its application. This research also provides a reference method for the study of growth-promoting mechanism of endophytic fungi.
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Data availability
Transcriptome data accession: SRR11308217; ITS gene accession: MT068424.
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Acknowledgements
We are grateful to National key R & D plan in the 13th five year plan (2016yfc50500300), Natural Science Foundation of Heilongjiang Province (C2016053, LH2020H093), Scientific research project of traditional Chinese medicine in Heilongjiang Province (general survey of traditional Chinese medicine resources 2018hljzyzypc-21), Project of "Support plan for young backbone teachers" of Heilongjiang University of traditional Chinese medicine (15041190011) for their financial support. We are very grateful to Annoroad for their technical support.
Funding
This work was supported by National key R & D plan in the 13th five year plan (2016yfc50500300), Natural Science Foundation of Heilongjiang Province (C2016053, LH2020H093), Scientific research project of traditional Chinese medicine in Heilongjiang Province (general survey of traditional Chinese medicine resources 2018hljzyzypc-21), Project of "Support plan for young backbone teachers" of Heilongjiang University of traditional Chinese medicine (15041190011).
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CD and ZW designed this research. KL, JL did a study on the growth-promoting effect of endophyticfungus, CD, JL and WS performed the related experimental research and analysis of transcriptome. CD and WS drafted the manuscript. All authors read and approved the final manuscript.
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Ding, C., Wang, S., Li, J. et al. Transcriptomic analysis reveals the mechanism of host growth promotion by endophytic fungus of Rumex gmelinii Turcz. Arch Microbiol 204, 443 (2022). https://doi.org/10.1007/s00203-022-03072-9
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DOI: https://doi.org/10.1007/s00203-022-03072-9