Abstract
In this study, a wild-type and five distinct rifampicin-resistant (Rifr) rpoB mutants of Pseudomonas stutzeri (i.e., Q518R, D521Y, D521V, H531R and I614T) ability were investigated against harsh environments (particularly nutritional complexity). Among these, the robust Rifr phenotype of P. Stutzeri was associated only with base replacements of the amino deposits. The use of carboxylic and amino acids significantly increased in various Rifr mutants than that of wild type of P. stutzeri. The assimilation of carbon and nitrogen (N) sources of Rifr mutants’ confirmed that the organism maintains the adaptation in nutritionally complex environments. Acetylene reduction assay at different times also found the variability for N-fixation in all strains. Among them, the highest nitrogenase activity was determined in mutant ‘D521V’. The assimilation of carbon and nitrogen sources of P. stutzeri and its Rifr mutants ensures that the organism maintains the adaptability in nutritionally complex environments through fixing more nitrogen.
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Data recorded in the current study are available in all Tables and Figures of the manuscript.
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Acknowledgements
The support of the Biotechnology Research Institute of the Chinese Academy of Agricultural Sciences for providing technical and other basic supports to carry out the study is duly acknowledged. The financial support of the NATP project, Bangladesh Agricultural Research Council (NATP phase-1, IDA credit 4386 BD). Also, the authors extend their appreciation to the Taif University Researches Supporting Project number (TURSP 2020/39), Taif University, Taif, Saudi Arabia.
Funding
The work was supported by the Biotechnology Research Institute of the Chinese Academy of Agricultural Sciences and the NATP project, Bangladesh Agricultural Research Council (NATP phase-1, IDA credit 4386 BD) as a Ph. D research grant. The current work was also partially funded by Taif University Researches Supporting Project number (TURSP 2020/39), Taif University, Taif, Saudi Arabia.
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Conceptualization: KMA, YY, ML and MAI; methodology: KMA, YY, ML and MAI; software: KMA and AH; validation: KMA, YY, ML and MAI; formal analysis: KMA and AH; investigation: KMA, YY, ML and MAI; resources: KMA and AH; data curation: KMA and AH; writing—original draft preparation, KMA, YY, ML, and MAI; writing—review and editing AG and AH; visualization: KMA, YY, ML, and MAI; supervision, YY and ML; project administration: KMA., AG and AH; funding acquisition: AG, YSA, AAA and AH. All authors have read and agreed to publish the current version of the manuscript.
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Communicated by Erko Stackebrandt.
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Alam, K.M., Yan, Y., Lin, M. et al. Insight rifampicin-resistant (rpoB) mutation in Pseudomonas stutzeri leads to enhance the biosynthesis of secondary metabolites to survive against harsh environments. Arch Microbiol 204, 437 (2022). https://doi.org/10.1007/s00203-022-03064-9
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DOI: https://doi.org/10.1007/s00203-022-03064-9