Abstract
We evaluated the effect of phytogenic additive Prosopis juliflora on populations of ruminal ciliated protozoa and its correlation with variables related to sheep nutrition. In this experiment, five cannulated adult Santa Ines ewes were submitted to the additive intake. Each animal received 6 mL of the extract daily. The experimental design adopted was the Latin square 5 × 5 (five concentrations of additive 0, 200, 400, 600 and 800 mg/mL of water and five periods of 18 days). The additive quadratically reduces the number of large, small and total ciliating protozoa, without promoting changes in the number of medium protozoa in the rumen. Among the genera investigated, Isotricha and Dasytricha were the only ones affected by supplementation. Additionally, the concentration of ruminal protozoa correlates significantly with the variables related to the nutrition of the animal. The effects of the additive on these variables were particularly pronounced at concentrations around 600 mg/mL.
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This study was funded by the Foundation for Science and Technology Support from Pernambuco (project number: APQ-1689-5.04/12).
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ERFF and RTAJ data curation, investigation, methodology, writing—original draft, and visualization. GRRC and CSS methodology, formal analysis, and writing—review/editing. JSL and KPP data curation, investigation, methodology, and visualization. EJOS and TRT conceptualization, data curation, formal analysis, investigation, methodology, project administration, resources, supervision, and writing—review/editing.
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This study was conducted in compliance with recommendations from the National Council for Animal Experimentation Control. The protocol was approved by the Animal Experimentation Ethics Committee of the Federal Rural University of Pernambuco, State of Pernambuco, Brazil (license number 005/2014).
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Communicated by Erko Stackebrandt.
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Feitoza, E.R.F., Alves Júnior, R.T., da Rocha Costa, G.R. et al. Phytogenic additive from Prosopis juliflora on populations of rumen ciliate protozoa and its correlation with nutrition of sheep. Arch Microbiol 204, 425 (2022). https://doi.org/10.1007/s00203-022-03041-2
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DOI: https://doi.org/10.1007/s00203-022-03041-2