Abstract
There are numerous factors restricting wide application of lactic acid bacteria (LAB) in dairy industry, causing urgent demands for novel bioprotectants. Protective effects and metabolites of Lactococcus lactis subsp. lactis (L. lactis) from ultraviolet (UV)-induced supernatant were investigated and the protective mechanism was explored. The strain viability of the group treated with the supernatant of continuous UV irradiation (V1) and the group with intermittent UV irradiation (V2) was 8.45 and 14.13 times of the control group, respectively. Further exploration on the protective of L. lactis supernatant, under different dose of UV treatment, showed it was dose-dependent. The condition for the supernatant with best protective effect was vertical distance 50.00 cm, horizontal distance 25.00 cm, intermittent UV irradiation (30 s interval 30 s) for 4.5 min (V2), which was chose for untargeted metabolite analysis. And that in V1 was for comparative study. There were 181 up-regulated metabolites in V1 and 161 up-regulated metabolites in V2, respectively. Most of the up-regulated metabolites were related to secondary metabolite synthesis, environmental microbial metabolism, antibiotic synthesis and amino acid biosynthesis. Notably, production of dithiothreitol (DTT) in V2 was 65.2-fold higher than that in the control group. Trehalose in ABC transporter pathway was also up-regulated in the metabolites induced by UV. Results indicated that L. lactis could adapt to the UV stress by adjusting metabolic pathways and producing special metabolites to protect itself. This research offers the basis for robust strain development and contributes to initial study on potential bioprotectant.
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Acknowledgements
This study was supported by the National Natural Science Foundation of China (81870093, 31900296), the Research Project of People’s Liberation Army, (BX115C007, BFP20C006), and the Natural Science Foundation of Henan Province for Outstanding Youth (202300410365), Key Scientific Research Projects of Higher Education Institutions in Henan Province (21A530006), the Program for Science & Technology Innovation Talents in Universities of Henan Province (22HASTIT037).
Funding
This work was funded by the National Natural Science Foundation of China (Grant nos. 81870093, 31900296); the Research Project of People’s Liberation Army (Grant nos. BX115C007, BFP20C006); Natural Science Foundation of Henan Province (Grant no. 202300410365); Key Scientific Research Projects of Higher Education Institutions in Henan Province (Grant no. 21A530006), the Program for Science & Technology Innovation Talents in Universities of Henan Province (Grant no. 22HASTIT037).
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YG, LC and LZ performed the experiments. JL, JZ, YG and LC conceived the study. Material preparation, data collection and analysis were performed by YG. YG wrote the paper. All authors read and approved the paper.
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Gao, Y., Zhu, J., Zhao, L. et al. Protective effect of the stressed supernatant from Lactococcus lactis subsp. lactis and its metabolic analysis. Arch Microbiol 204, 428 (2022). https://doi.org/10.1007/s00203-022-03034-1
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DOI: https://doi.org/10.1007/s00203-022-03034-1