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Genome-based reclassification of Streptococcus ursoris as a later heterotypic synonym of Streptococcus ratti

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Abstract

Among the species of genus Streptococcus two members namely Streptococcus ratti and Streptococcus ursoris were isolated from oral cavity of rat and bear, respectively. Type strain of these members shows a 16S rRNA gene sequence similarity of 98.9%. Based on systematic phylo-taxonogenomics investigations, we could deduce the taxonomic assignment of the members of these species. Genome similarity assessment among the type strain of these members using average nucleotide identity (orthoANI and fastANI), digital DNA–DNA hybridization and average amino acid identity (AAI) were 98.5, 98.3, 88, and 98.3% respectively. All these values exceed the species delineation cutoffs suggesting a unified species. Phylogenetic tree obtained using 16S rRNA gene sequence also indicates the monophyletic nature of the member strains. Such monophyletic taxonomic positioning of the strains was further complemented with the whole genome-based phylogenomic tree. Based on these evidences, we propose S. ursoris should be reclassified as a later heterotypic synonym of S. ratti.

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Abbreviations

CSI:

Conserved sequence indels

ANI:

Average Nucleotide Identity

dDDH:

Digital DNA–DNA hybridization

ML:

Maximum likelihood

GS:

Genomospecies

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Acknowledgements

Authors convey a special thanks to Dr. Prabhu Patil, CSIR-IMTECH, Chandigarh for their immense support throughout the whole study. Authors also convey their gratitude towards the critical inputs from Dr. Kanika Bansal, CSIR-IMTECH, Chandigarh for her suggestion on the manuscripts.

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The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Authors and Affiliations

  1. School of Biotechnology, Gangadhar Meher University, Sambalpur, Odisha, 768004, India

    Sanjeet Kumar & Santosh K. Sethi

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  1. Sanjeet Kumar
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  2. Santosh K. Sethi
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Contributions

SK have performed the analysis and drafted manuscript with the inputs from SKS. SKS have supervised the study by performing data correction, formal analysis and drafting of methodology.

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Correspondence to Santosh K. Sethi.

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All the authors declare no conflict of interest.

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This is an observational study which does not require any ethical approval.

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Communicated by Erko Stackebrandt.

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Kumar, S., Sethi, S.K. Genome-based reclassification of Streptococcus ursoris as a later heterotypic synonym of Streptococcus ratti. Arch Microbiol 204, 405 (2022). https://doi.org/10.1007/s00203-022-03012-7

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  • DOI: https://doi.org/10.1007/s00203-022-03012-7

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