Abstract
Honeybee products have been among important consumer products throughout history. Microbiota has attracted attention in recent years due to both their probiotic value and industrial potential. Fructophilic lactic acid bacteria (FLAB), whose field of study has been expanding rapidly in the last 20 years, are among the groups that can be isolated from the bee gut. This study aimed to isolate FLAB from the honeybees of two different geographic regions in Turkey and investigate their probiotic, metabolic and anti-quorum sensing (anti-QS) potential. Metabolic properties were investigated based on fructose toleration and acid and diacetyl production while the probiotic properties of the isolates were determined by examining pH, pepsin, pancreatin resistance, antimicrobial susceptibility, and antimicrobial activity. Anti-QS activities were also evaluated with the Chromobacterium violaceum biosensor strain. Two FLAB members were isolated and identified by the 16S rRNA analysis as Fructobacillus tropaeoli and Apilactobacillus kunkeei, which were found to be tolerant to high fructose, low pH, pepsin, pancreatin, and bile salt environments. Both isolates showed anti-QS activity against the C. violaceum biosensor strain and no diacetyl production. The daily supernatants of the isolates inhibited the growth of Enterococcus faecalis ATCC 29212 among the selected pathogens. The isolates were found resistant to kanamycin, streptomycin, erythromycin, and clindamycin. In the evaluation of the probiotic potential of these species, the negative effect of antibiotics and other chemicals to which honeybees are directly or indirectly exposed draws attention within the scope of the “One Health” approach.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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The idea was conceptualized by DS and Material preparation, data collection, and data analysis were performed by DS, KCT, MG, and MEK. The first draft of the manuscript was prepared by DS, KCT, MG, and MEK. The manuscript was reviewed and edited by DS, KCT, MG, MEK, and NA. Supervision was provided by NA.
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Simsek, D., Kiymaci, M.E., Tok, K.C. et al. Investigation of the probiotic and metabolic potential of Fructobacillus tropaeoli and Apilactobacillus kunkeei from apiaries. Arch Microbiol 204, 432 (2022). https://doi.org/10.1007/s00203-022-03000-x
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DOI: https://doi.org/10.1007/s00203-022-03000-x