Abstract
Human microbiome studies have shown diversity to exist among different ethnic populations. However, studies pertaining to the microbial composition of CRC among the Indian population have not been well explored. We aimed to decipher the microbial signature in tumor tissues from North Indian CRC patients. Next-generation sequencing of tumor and adjacent tissue-derived bacterial 16S rRNA V3-V4 hypervariable regions was performed to investigate the abundance of specific microbes. The expression profile analysis deciphered a decreased diversity among the tumor-associated microbial communities. At the phyla level, Proteobacteria was differentially expressed in CRC tissues than adjacent normal. Further, DeSeq2 normalization identified 4 out of 79 distinct species (p < 0.005) only in CRC, Bacteroides massiliensis, Alistipes onderdonkii, Bifidobacterium pseudocatenulatum, and Corynebacterium appendicis. Thus, the findings suggest that microbial signatures can be used as putative biomarkers in diagnosis, prognosis and treatment management of CRC.
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Acknowledgements
The authors would like to thank Parul Chugh (SGRH) for statistical help and the co-workers at Department of Research, SGRH.
Funding
This study was supported by the grant provided by Research and Development program (RDP Grant No.: 4.9.36-011), Sir Ganga Ram Hospital (SGRH), India.
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All authors contributed towards writing the manuscript and have given approval to the final version of the manuscript. RH performed collection of human samples/isolation/NGS assays and framing the manuscript. SB guided, framed the manuscript, inferenced the data and edited the manuscript. RR performed the metagenomics data analysis and prepared the figures. SR enrolled the patients as per inclusion criteria, provided the clinical samples and demographics of the patients. DB contributed in manuscript proof reading. PN & NKS assisted in experimental standardization and NGS data acquisition and cleaning. SC conceptualized, guided, analyzed the data, framed and edited the manuscript.
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This study was reviewed and approved by Sir Ganga Ram Hospital Ethics Committee (Ethics approval code: EC/06/16/1000). The patients/participants provided their informed consent to participate in this study.
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Communicated by Erko Stackebrandt.
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Hasan, R., Bose, S., Roy, R. et al. Tumor tissue-specific bacterial biomarker panel for colorectal cancer: Bacteroides massiliensis, Alistipes species, Alistipes onderdonkii, Bifidobacterium pseudocatenulatum, Corynebacterium appendicis. Arch Microbiol 204, 348 (2022). https://doi.org/10.1007/s00203-022-02954-2
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DOI: https://doi.org/10.1007/s00203-022-02954-2