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Exploring plant growth-promoting rhizobacteria as stress alleviators: a methodological insight

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Abstract

Rhizospheric and root-endophyte bacteria can stimulate plant growth through diverse biochemical mechanisms and pathways, particularly under biotic and abiotic stresses. For this reason, biotechnological trends on plant growth-promoting rhizobacteria (PGPR) application as biofertilizers, bioremediators, and stress alleviators are gaining increasing interest as ecofriendly strategies for sustainable agriculture management and soil restoration. The first steps needed to implement these technologies are isolation, screening, and characterization of PGPR that can be potentially applied as bioinoculants to alleviate biotic and/or abiotic stresses. Therefore, a complete and accurate methodological study and laboratory techniques are required to warrant the correct achievement of these steps. This review compiles and details the fundamentals, methods, and procedures of key protocols used in isolation and characterization of PGPR for plant stress alleviation.

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Acknowledgements

We highly appreciate the helpful comments made by the editors and reviewers.

Funding

We thank Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT, PICT 2016–2060, Della Mónica IF) and Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET) for funding this work.

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Author notes

  1. Ivana F. Della Mónica and Arnoldo Wong Villarreal have contributed equally to this work.

Authors and Affiliations

  1. Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales. Instituto de Micología y Botánica, Consejo Nacional de Investigaciones Científicas y Tecnológicas (INMIBO-CONICET), Universidad de Buenos Aires (UBA), Lab. 69, 4 Piso, Pabellón II, Ciudad Universitaria, Intendente Güiraldes 2160, 1428, Buenos Aires, Argentina

    Ivana F. Della Mónica & Pablo J. Stefanoni Rubio

  2. División Agroalimentaria, Universidad Tecnológica de la Selva, Ocosingo, 29950, Chiapas, Mexico

    Arnoldo Wong Villarreal

  3. Laboratorio de Edafología y Ambiente, Facultad de Ciencias, Universidad Autónoma del Estado de México, 50000, Toluca, Estado de México, México

    Rocío Vaca-Paulín & Gustavo Yañez-Ocampo

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  1. Ivana F. Della Mónica
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Contributions

Original Idea: YOG and DMIF. Literature search: DMIF, YOG, SRP, WA, and RVP. Writing—Original draft: DMIF, YOG, SRP, and WA, and critical review and Editing: DMIF and YOG. All authors read and approved the final manuscript.

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Correspondence to Ivana F. Della Mónica or Gustavo Yañez-Ocampo.

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Della Mónica, I.F., Wong Villarreal, A., Stefanoni Rubio, P.J. et al. Exploring plant growth-promoting rhizobacteria as stress alleviators: a methodological insight. Arch Microbiol 204, 316 (2022). https://doi.org/10.1007/s00203-022-02909-7

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