Abstract
A bacterium, designated as KMU-166T, belonging to the class Gammaproteobacteria, was isolated from seawater collected on the coastline of Dadaepo, Republic of Korea. Strain KMU-166T was Gram-staining-negative, ovoid-shaped, motile, strictly aerobic, beige-colored, catalase-positive, and oxidase-negative; and had a chemoorganoheterotrophic metabolism. The novel isolate was found to grow at 1–4% NaCl concentrations (w/v), pH 6.5–9.5, and 10–40 °C. The 16S rRNA gene sequence-based phylogeny showed that strain KMU-166T affiliates to the family Spongiibacteraceae and that it shared the greatest sequence similarity (96.4%) with Spongiibacter marinus HAL40bT. The main (> 10%) cellular fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c), C17:1ω8c, and C18:1ω7c. The predominant respiratory quinone was ubiquinone-8 (Q-8). Strain KMU-166T comprised phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and two unidentified lipids. The assembled draft genome was 4.40 Mbp in size with a DNA G+C content of 55.7%. The average nucleotide identity, digital DNA–DNA hybridization, and average amino acid identity values of KMU-166T and Spongiibacter marinus HAL40bT, Spongiibacter tropicus CL-CB221T, and “Spongiibacter pelagi” KMU-158T were found to be 77.7–79.8%, 13.7–15.4%, and 66.1–70.9%, respectively. From the distinguishable polyphasic taxonomic results obtained, the strain is considered to represent a novel species of the genus Spongiibacter for which the name Spongiibacter thalassae sp. nov. is proposed. The type strain of S. thalassae sp. nov. is KMU-166T (= KCCM 90449T = NBRC 114308T).
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Supplementary Fig. 1
Transmission electron micrograph of a negatively stained cell of strain KMU-166T. Bar, 500 nm (PDF 918 KB)
Supplementary Fig. 2
A two-dimensional thin-layer chromatogram showing the total polar lipid compositions of KMU-166T and reference strains. Total polar lipids were detected by spraying the plate with molybdatophosphoric acid. PE: phosphatidylethanolamine, PG: phosphatidylglycerol, DPG: diphosphatidylglycerol, PL: unidentified phospholipid, L: unidentified lipid (PDF 137 KB)
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Yoon, J. Spongiibacter thalassae sp. nov., a marine gammaproteobacterium isolated from seawater. Arch Microbiol 204, 273 (2022). https://doi.org/10.1007/s00203-022-02888-9
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DOI: https://doi.org/10.1007/s00203-022-02888-9