Abstract
This study aimed to evaluate the differences in the characteristics of extracellular polymeric substances (EPSs) secreted by Mycobacterium gilvum SN12 (M.g. SN12) cultured on pyrene (Pyr) and benzo[a]pyrene (BaP). A heating method was used to extract EPSs from M.g. SN12, and the composition, emulsifying activity, and morphology of EPS extracts were investigated. Results showed that EPS extracts varied significantly with Pyr or BaP addition to the bacterial cultures. The concentration of proteins and carbohydrates, the main components of the EPS extracts, first increased and then decreased, with an increase in the concentration of Pyr (0–120 mg L−1) and BaP (0–120 mg L−1). A similar trend was observed for the emulsifying activity of the EPS extracts. EPSs extracted from all cultures exhibited a compact structure with a smooth surface, except for EPSs extracted from BaP-grown M.g. SN12, which revealed a more fragile and softer surface. These findings suggest that Pyr and BaP had different influences on the properties of isolated EPSs, providing insights into the mechanism underlying polycyclic aromatic hydrocarbons (PAHs) biodegradation by some EPS-secreting bacteria. To the best of our knowledge, this is the first report on the texture profile of EPS samples extracted from M.g. SN12 grown on PAHs.
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Acknowledgements
We would like to express my gratitude to all those who helped me during the writing of this manuscript. We gratefully acknowledge Associate Professor Liping Weng for her comments and suggestions. We appreciated Xinyan Li for her help during the cultivation of bacteria. Finally, we thank the financial support from all the funder mentioned in the funding.
Funding
This work was supported by the Natural Science Foundation of China (No. 41673132), Natural Science Foundation of Liaoning Province of China (No. 2021-MS-027), Innovation Program in Sciences and Technologies for Young and Middle-aged Scientists (No. RC200123).
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CJ carried out the experiment and was a major contributor in writing the manuscript. CL took part in the design of the investigation and analysis of collected samples. ZG took part in studying three-dimensional excitation and emission matrix fluorescence of extracellular polymeric substance (EPS) extracts. XL took part in the data interpretation and writing the manuscript. ZN developed the characterisation of EPS extracts. All the authors read and approved the final manuscript.
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Jia, C., Liu, C., Gong, Z. et al. Differences in the properties of extracellular polymeric substances responsible for PAH degradation isolated from Mycobacterium gilvum SN12 grown on pyrene and benzo[a]pyrene. Arch Microbiol 204, 227 (2022). https://doi.org/10.1007/s00203-022-02849-2
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DOI: https://doi.org/10.1007/s00203-022-02849-2