Abstract
A novel lytic Enterococcus faecalis phage, EFC1, was isolated from the sewage of a farm in Handan, China, and its genome was analyzed and described. The phage could infect 87.5% of the chicken-derived Enterococcus faecalis preserved in our laboratory. The genome of phage EFC1 consists of a circular double-stranded DNA with a length of 56,099 bp and a G + C content of 39.96%, containing 89 predicted protein-coding genes as well as 2 tRNAs, which are involved in phage intron, structure, transcription, packaging, DNA replication, modification, cell lysis, and other functions, indicating the genetic and functional characteristics of this phage. Genome comparison analysis revealed that phage EFC1 can be regarded as new genus Saphexavirus phage in the Siphoviridae family.
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Acknowledgements
This study received the support of the Department of science and technology of Hebei Province, China and the school of life sciences and food engineering, Hebei University of Engineering, China. Thanks to the Electron Microscopy Experimental Center of Hebei Medical University for providing equipment and guidance training. The library preparation, sequencing and assembly, and annotation guidance are all supported and guided by GENEWIZ Technology Co., Ltd.
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This study was funded by Special project for tackling key technical problems of science and Technology Department of Hebei Province (19226609D).
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This article does not contain any studies with human participants or animals performed by any of the authors.
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The GenBank accession number for phage EFC1 is MW677132.
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Communicated by Erko Stackebrandt.
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Wang, Q., Liu, N. Complete genome analysis of bacteriophage EFC1 infecting Enterococcus faecalis from chicken. Arch Microbiol 204, 413 (2022). https://doi.org/10.1007/s00203-022-02838-5
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DOI: https://doi.org/10.1007/s00203-022-02838-5