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Organophosphorus insecticides mineralizing endophytic and rhizospheric soil bacterial consortium influence eggplant growth-promotion

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Abstract

This study was aimed to evaluate eggplant's growth-enhancing activity of chlorpyrifos and diazinon-degrading endophytic and rhizospheric soil bacteria isolated from cauliflower and tomato roots and the rhizospheric soil of rice roots, respectively. The identified endophytes belong to the AcinetobacterEnterobacter and Klebsiella genera, while rhizospheric soil isolates belong to PantoeaAcinetobacterKosakoniaMorganellaEnterobacter, and Klebsiella genera with species variation and genetic distances. All the strain’s consumed 100% (50 mg/5 mL) chlorpyrifos and diazinon after 14 days of exposure, except for Pantoea sp. HSTU-Sny4 (84%) and Kosakonia sp. HSTU-ASn39 (42%). The strain’s exhibited N-fixation, P-solubilization, indole-3-acetic acid (IAA), and ACC-deaminase production capabilities. The individual strain’s and consortium treatment enhanced eggplant growth at germination, seedling, vegetative and reproductive stages. Plant growth-promoting genes, e.g., nif-cluster, chemotaxis, phosphates, sulfur, abiotic stress, chemotaxis, biofilm formation and organophosphorus insecticide-degrading genes were annotated in Klebsiella sp. HSTU-Sny5 and Morganella sp. HSTU-ASny43 genomes. Importantly, the mixed consortium supplemented with 40% urea-treated eggplants demonstrated similar growth parameters compared to the 100% urea eggplants. Plenty of insecticide-degrading proteins belonged to HSTU-Sny5 and HSTU-ASny43 strain’s and had interacted with 100 different insecticides as confirmed in virtual screening. This research has a significant role in reducing the application of chemical fertilizer and bioremediation of pesticides in agriculture.

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Data availability

The sequence data reported in this study have been deposited in the NCBI database. The 16S rRNA gene sequences of the strains were deposited in the NCBI database with accession numbers MN611130-MN611134, MN538301, MK696217-MK696220, MK656938, MK656939-MK656941, MW408122, MN559048, MN656942, MN795724, MN795725, and MN559049. The whole-genome datasets generated in this manuscript can be found in NCBI under the Klebsiella sp. HSTU-Sny5 strain (BioSample: PRJNA580343, BioProject SAMN13156565, Accession number WLCL00000000) Klebsiella sp. HSTU-Sny5, and Morganella sp. (BioSample: PRJNA592769, BioProject: SAMN13439433, and accession number WSPG00000000).

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Acknowledgements

We thank all staff of plant pathology laboratory, agricultural chemistry laboratory of agriculture faculty, HSTU for their assistance during plant and bacterial growths. In addition, the authors are thankful to Mr. Md. Ashikujjaman Ashik for his assistance in protein model analysis.

Funding

The research was supported by the Bangladesh University Grants Commission (BUGC) for grants number Biology-17/2018/3432. The authors are also thankful to The World Academy of Sciences (TWAS), Trieste, Italy for their initial supports to Dr. Md. Azizul Haque as an individual young scientist research grant (No. 17–475 RG/BIO/AS_I), and the Ministry of Science and Technology (MOST), Bangladesh.

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Contributions

SRD: Investigation & Thesis preparation; AA: GC–MS analysis and interpretation; MAM & GCD: Investigation, Genomics & Bioinformatics analysis; MSH & ZH: Investigation, Data curation, and Analysis; AR & MAH: Data curation, and critically revised the manuscript; SY: Analysis, Experiment; MAI, MNE-NN, KMC: Resources; MAH: Conceptualization, Project administration, Supervision, Funding acquisition, Methodology, Experiment, Analysis, Writing—review & editing.

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Correspondence to Md. Azizul Haque.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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The manuscript has not been submitted elsewhere and is not being considered by any other journal. All authors have approved to submit the manuscript in the journal of “Archives of Microbiology”.

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Communicated by Erko Stackebrandt.

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Das, S.R., Haque, M.A., Akbor, M.A. et al. Organophosphorus insecticides mineralizing endophytic and rhizospheric soil bacterial consortium influence eggplant growth-promotion. Arch Microbiol 204, 199 (2022). https://doi.org/10.1007/s00203-022-02809-w

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  • DOI: https://doi.org/10.1007/s00203-022-02809-w

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