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Allobacillus salarius sp. nov., and Allobacillus saliphilus sp. nov., isolated from shrimp paste (ka-pi) in Thailand

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Abstract

Two strains of moderately halophilic, Gram-stain-positive and spore-forming rods, designated as SKP4-8T and SKP8-2T isolated from shrimp paste (Ka-pi), were taxonomic studied based on polyphasic approach. Strain SKP4-8T grew at pH 6.0–9.0 (optimum 7.0), at 25–45 °C (optimum 37 °C) and in 1–16% (w/v) NaCl (optimum 5–10%). Strain SKP8-2T grew at pH 6.0–9.0 (optimum 8.0), at 25–45 °C (optimum 37 °C) and in 0–20% (w/v) NaCl (optimum 3–10%). The strains contained meso-diaminopimelic acid in cell-wall peptidoglycan and the major menaquinone was MK-7. Strain SKP4-8T contained iso-C15:0, anteiso-C15:0 and iso-C17:0; and strain SKP8-2T contained anteiso-C15:0, iso-C15:0, iso-C16:0 and antesio-C17:0 as major cellular fatty acids. Phosphatidylglycerol, diphosphatidylglycerol, unknown phospholipids and an unknown glycolipid were detected as major polar lipids. On the basis of 16S rRNA gene sequence analysis, strains SKP4-8T and SKP8-2T belonged to the genus Allobacillus and were closely related to Allobacillus halotolerans LMG 24826T with 98.8% and 99.3% similarity, respectively. The comparative genome analysis based on average nucleotide identity (ANI) and digital DNA–DNA hybridization revealed that both strains showed the values below 95 and 70%, from each other and from Allobacillus halotolerans LMG 24826T, respectively. Based on the data from this polyphasic study, strains SKP4-8T and SKP8-2T represent novel species of the genus Allobacillus and the name Allobacillus salarius sp. nov. was proposed for SKP4-8T (= KCTC 33905T = LMG 30016T = TISTR 2499T); and Allobacillus saliphilus sp. nov. for SKP8-2T (= KCTC 33906T = LMG 29682T = TISTR 2558T).

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Funding

This study was supported by the Grant for International Research Integration: Research Pyramid, Ratchadaphiseksomphot Endowment Fund (GCURP_58_01_33_01), Chulalongkorn University and the International Partnership Program of Chinese Academy of Sciences (Grant No. 153211KYSB 201900211). Additional thanks to the Ratchadapiseksomphot Endowment Fund, Chulalongkorn University for a post-doctoral fellowship to P. K.

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Authors and Affiliations

  1. Biotechnology Program and Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Supalurk Yiamsombut & Ancharida Savarajara

  2. Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand

    Pawina Kanchanasin, Wongsakorn Phongsopitanun, Nattakorn Kuncharoen & Somboon Tanasupawat

  3. World Data Center for Microorganisms (WDCM), Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, People’s Republic of China

    Wenyu Shi, Linhuan Wu & Juncai Ma

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  1. Supalurk Yiamsombut
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  2. Pawina Kanchanasin
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  3. Wongsakorn Phongsopitanun
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  4. Nattakorn Kuncharoen
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  9. Somboon Tanasupawat
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Correspondence to Somboon Tanasupawat.

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Communicated by Erko Stackebrandt.

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Yiamsombut, S., Kanchanasin, P., Phongsopitanun, W. et al. Allobacillus salarius sp. nov., and Allobacillus saliphilus sp. nov., isolated from shrimp paste (ka-pi) in Thailand. Arch Microbiol 204, 71 (2022). https://doi.org/10.1007/s00203-021-02694-9

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