Abstract
A Gram-stain-negative, pink-pigmented, aerobic, non-motile and rod-shaped bacterium, designated as strain H15T, was isolated from Acmaea sp., collected from Weihai, Shandong Province, China. The novel isolate was able to grow at 4–37 °C (optimum 33 °C), pH 5.5–9.0 (optimum 7.0) and with 0.0–7.0% NaCl (optimum 4%, w/v). Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that the strain belonged to the family Rhodobacteraceae and was associated to the type strain of Pseudaestuariivita atlantica (96.7%). Genome analysis showed that the genome size was 3,893,398 bp and the DNA G + C content obtained from the draft genome sequence was 56.7%. The secondary metabolites predicated that the strain H15T contained one cluster of lasso peptide, one cluster of bacteriocin, two clusters of terpene production, two clusters of homoserine lactone and one cluster of beta lactone. The average amino acid identity, average nucleotide identity and digital DNA–DNA hybridization values between genome sequences of strain H15T and all the related strains compared were lower than 63.1, 72.0 and 19.7%, respectively. Based on the analysis of chemical components, the predominant cellular fatty acids were summed featured 8 (C18:1ω7c/ω6c, 46.1%), C20:1 ω7c (17.1%), the major polar lipids contained phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and an unidentified lipid and the predominant menaquinone was Q10. Therefore, the combined chemotaxonomic, phenotypic and phylogenetic data indicated that the strain was considered to represent a novel species of the genus Pseudaestuariivita and the name Pseudaestuariivita rosea sp. nov. was proposed for strain H15T (MCCC 1K04420T = KCTC 82505T).
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Abbreviations
- AAI:
-
Average amino acid identity
- ANI:
-
Average nucleotide identity
- cAAI:
-
Core-gene average amino acid identity
- COG:
-
Cluster of orthologous group
- dDDH:
-
Digital DNA–DNA hybridization;
- GGDC:
-
Genome-to-genome distance calculator
- HPLC:
-
High performance liquid chromatography
- KCTC:
-
Korean collection for type cultures
- KEGG:
-
Kyoto encyclopedia of genes and genomes
- LPS:
-
Lipopolysaccharide
- MA:
-
Marine agar 2216
- MB:
-
Marine broth 2216
- MCCC:
-
Marine culture collection of China
- MEGA:
-
Molecular evolutionary genetics analysis
- NCBI:
-
National Center for Biotechnology Information
- PC:
-
Phosphatidylcholine
- PE:
-
Phosphatidylethanolamine
- PG:
-
Phosphatidylglycerol
- POCP:
-
Percent of conserved proteins
- TLC:
-
Two-dimensional thin-layer chromatogram
- VFDB:
-
Virulence factors of pathogenic bacteria
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Acknowledgements
We thank Prof. Aharon Oren for his valuable help with naming the species.
Funding
This work was supported by the National Natural Science Foundation of China (31700116), the Natural Science Foundation of Shandong Province (ZR2017MC019), the China Postdoctoral Science Foundation (2017M62218), and the Key Science and Technology Program of Weihai (1070413421511).
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YJY contributed to the sample collection and biochemical characterization. STY analysed the data and drafted the manuscript. ZC contributed to the construction of phylogenetic tree. MJZ contributed to revision of this article. SKG contributed to genome submission and analyses. YXZ designed the experiments and revised the manuscript.
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Yun, ST., Chen, Z., Yi, YJ. et al. Pseudaestuariivita rosea sp. nov., isolated from Acmaea sp., a marine mollusk. Arch Microbiol 204, 94 (2022). https://doi.org/10.1007/s00203-021-02690-z
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DOI: https://doi.org/10.1007/s00203-021-02690-z