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Partial characterization of viscous exopolymer produced by Alkalihalobacillus sp. strain SB-D (KJ372395) with emulsification and adhesive properties

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Abstract

Twenty-two alkaliphilic bacteria were isolated from spent waters of an agrochemical factory, Goa, India, and screened for exopolymer (EP) production on Horikoshi I/II media. Strain SB-D produced highly viscous cell-bound and extracellular EP quantified by alcian blue adsorption assay. The 16S rRNA gene sequence of strain SB-D showed 97.34% similarity to Alkalihalobacillus lehensis strain MLBT. The strain maximally produced EP (10.3 g/L) at pH 10.3, 25 °C after 16 h growth on shaker, followed by 4 h at static condition with 4% inoculum in the presence of 3% glucose and 0.1% yeast extract. The EP precipitated using isopropanol contained carbohydrates (212 µg/mg), lipids (130 µg/mg), and proteins (86.16 µg/mg) as major components. Further, gas chromatography detected the presence of d-glucose, d-arabinose, d-mannose, d-galactose, d-xylose and d-ribose. Preliminary studies on EP characterization revealed emulsification and adhesive properties of the exopolymer.

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Acknowledgements

The authors are grateful to the Managing Director of Zuari Industries Ltd., Goa, India, for permission granted to collect alkaline spent water samples from the industry. Author S. Borkar acknowledges the assistance rendered by Dr. Neha Prabhu and Mr. Sanket Gaonkar in alignment of figures in the MS. The authors also thank Mr. Areef Sardar for SEM analysis and Fraddry D'souza, NIO, Goa, for GC analysis.

Funding

University Grants Commission (UGC), New Delhi, India, Project No. 23-113/99(WRO).

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Authors and Affiliations

  1. Department of Microbiology, Goa University, Taleigao Plateau, Goa, India

    Sunita R. Borkar & Saroj N. Bhosle

  2. UG and PG Department of Microbiology, P.E.S’s R.S.N College of Arts and Science, Farmagudi, Ponda, Goa, India

    Sunita R. Borkar

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  1. Sunita R. Borkar
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  2. Saroj N. Bhosle
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SRB designed, performed the experiments, analyzed the data, and drafted the manuscript. SNB helped in the data analysis and corrected the manuscript.

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Correspondence to Sunita R. Borkar.

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Author SRB dedicates this research work to her late mother Mrs. Nirmala Gupta.

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Communicated by Erko Stackebrandt.

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Borkar, S.R., Bhosle, S.N. Partial characterization of viscous exopolymer produced by Alkalihalobacillus sp. strain SB-D (KJ372395) with emulsification and adhesive properties. Arch Microbiol 204, 48 (2022). https://doi.org/10.1007/s00203-021-02679-8

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