Abstract
Murraya paniculata (L.) Jack is commonly cultivated as ornamental plant in Assam and has been used as spice and phytomedicine traditionally for many healthcare purposes. The therapeutic potential and chemical constituents of the essential oil of M. paniculata leaf was investigated against several pathogenic microbial species and human cancer cell lines. 29 chemical compounds were identified by GC–MS analysis from the essential oil representing 97.62% of the oil. The major compound identified was caryophyllene (20.93%). Leaf essential oil exhibited promising antibacterial activity against Mycobacterium smegmatis (MIC = 4 µg/mL) and Pseudomonas aeruginosa (MIC = 4 µg/mL). Best anticancer activity of the oil was observed for HeLa cells (IC50 = 6.28 μg/mL). Further, scanning electron microscopic studies revealed that the oil kills micro-organisms with the deformation of cellular morphology on treatment of the oil. Thus, the essential oil of M. paniculata leaf can be an excellent alternative for development of new antimicrobials and anticancer chemotherapeutic agents for the pharmaceutical industries.
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References
Abrahams KA, Besra GS (2018) Mycobacterial cell wall biosynthesis: a multifaceted antibiotic target. Parasitology 145(2):116–133. https://doi.org/10.1017/S0031182016002377
Abreu AC, Mc Bain AJ, Simoes M (2012) Plants as source of new antimicrobials and resistance-modifying agents. Nat Prod Rep 29(9):1007–1021. https://doi.org/10.1039/C2NP20035J
Adams RP (1995) Identification of essential oil components by gas chromatography/mass spectrometry. Allured Publishing Corporation, Carol Stream
Akula R, Ravishankar GA (2011) Influence of abiotic stress signals on secondary metabolites in plants. Plant Signal Behav 6(11):1720–1731. https://doi.org/10.4161/psb.6.11.17613
Boira H, Blanquer A (1998) Environmental factors affecting chemical variability of essential oils in Thymus piperella L. Biochem Syst Ecol 26(8):811–822. https://doi.org/10.1016/S0305-1978(98)00047-7
Bouyahya A, Dakka N, Talbaoui A, Et-Touys A, El-Boury H, Bakri AJ, Y, (2017) Correlation between phenological changes, chemical composition and biological activities of the essential oil from Moroccan endemic Oregano (Origanum compactum Benth). Ind Crops Prod 108:729–737. https://doi.org/10.1016/j.indcrop.2017.07.033
CDC, Antibiotic resistance threats in the United States, Atlanta (GA): U.S. Department of Health and Human Services (2019). https://www.cdc.gov/drugresistance/Biggest-Threats.html. Accessed on 3 Nov 2020
Chadwick M, Trewin H, Gawthrop F, Wagstaff C (2013) Sesquiterpenoids lactones: benefits to plants and people. Int J Mol Sci 14(6):12780–12805. https://doi.org/10.3390/ijms140612780
Chan WK, Tan LT, Chan KG, Lee LH, Goh BH (2016) Nerolidol: a sesquiterpene alcohol with multi-faceted pharmacological and biological activities. Molecules 21(5):529. https://doi.org/10.3390/molecules21050529
Chassagne F, Samarakoon T, Porras G, Lyles JT, Dettweiler M, Marquez L, Salam AM, Shabih S, Farrokhi DR, Quave CL (2021) A systematic review of plants with antibacterial activities: a taxonomic and phylogenetic perspective. Front Pharmacol 11:586548. https://doi.org/10.3389/fphar.2020.586548
Choudhury M (2015) Distribution and ex-situ conservation of plants, few observations from Assam state zoo, Guwahati, Assam. J Sci 5:361–365
Chowdhury JU, Bhuiyan MNI, Yusuf M (2008) Chemical composition of the leaf essential oils of Murraya koenigii (L) Spreng and Murraya paniculata (L) Jack, Bangladesh. J Pharmacol 3(2):59–63. https://doi.org/10.3329/bjp.v3i2.841
Clinical and Laboratory Standards Institutes (CLSI) (2012) Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically, Approved Standard-Ninth Edition; M07-A9.
Dahham SS, Tabana YM, Iqbal MA, Ahamed MB, Ezzat MO, Majid AS, Majid AM (2015) The anticancer, antioxidant and antimicrobial properties of the sesquiterpene β-caryophyllene from the essential oil of Aquilaria crassna. Molecules 20(7):11808–11829. https://doi.org/10.3390/molecules200711808
Dosoky NS, Satyal P, Gautam TP, Setzer WN (2016) Composition and biological activities of Murraya paniculata (L) Jack essential oil from Nepal. Medicines (basel) 3(1):7. https://doi.org/10.3390/medicines3010007
Garcia-Rubio R, de Oliveira HC, Rivera J, Trevijano-Contador N (2020) The fungal cell wall: Candida, Cryptococcus, and Aspergillus species. Front Microbiol 10:2993. https://doi.org/10.3389/fmicb.2019.02993
Gautam MK, Gangwar M, Nath G, Rao CV, Goel RK (2012) In-vitro antibacterial activity on human pathogens and total phenolic, flavonoid contents of Murraya paniculata Linn. leaves. Asian Pac J Trop Biomed 2(3):S1660–S1663. https://doi.org/10.1016/S2221-1691(12)60472-9
Hui LM, Zhao GD, Zhao JJ (2015) δ-Cadinene inhibits the growth of ovarian cancer cells via caspase-dependent apoptosis and cell cycle arrest. Int J Clin Exp Pathol 8(6):6046–6056
Kovats E (1965) Gas chromatographic characterization of organic substances in the retention index system. Adv Chromatogr 1:229–247
Li Q, Zhu LF, But PPH, Kong YC, Chang HT, Waterman PG (1988) Monoterpene and sesquiterpene rich oils from the leaves of Murraya species: chemotaxonomic significance. Biochem Syst Ecol 16(5):491–494. https://doi.org/10.1016/0305-1978(88)90050-6
Magrys A, Olender A, Tchorzewska D (2021) Antibacterial properties of Allium sativum L. against the most emerging multidrug-resistant bacteria and its synergy with antibiotics. Arch Microbiol. https://doi.org/10.1007/s00203-021-02248-z
Moo CL, Yang SK, Osman MA et al (2020) Antibacterial activity and mode of action of β-caryophyllene on Bacillus cereus. Pol J Microbiol 69(1):1–6. https://doi.org/10.33073/pjm-2020-007
National Committee for Clinical Laboratory Standards (NCCLS) (1999) Performance standards for antimicrobial susceptibility testing M100-S9
Neipihoi NB, Saikia S, Saikia S, Tamuli KJ, Bordoloi M (2020) Anticancer compounds from Croton caudatus Giesel and Eurya acuminata DC: traditional edible medicinal plants of Kuki Tribe. Nat Prod Res. https://doi.org/10.1080/14786419.2020.1815737
Neu HC, Gootz TD (1996) Antimicrobial chemotherapy, medical microbiology, 4th edn. University of Texas Medical Branch at Galveston, Galveston, pp 1–15
Ng MK, Abdulhadi-Noaman Y, Cheah YK, Yeap SK, Alitheen NB (2012) Bioactivity studies and chemical constituents of Murraya paniculata (Linn.) Jack. Int Food Res J 19(4):1307–1312
Olawore NO, Ogunwande IA, Ekundayo O, Adeleke KA (2004) Chemical composition of the leaf and fruit essential oils of Murraya paniculata (L.) Jack (Syn Murraya exotica Linn.). Flavour Fragr J 20(1):54–56. https://doi.org/10.1002/ffj.1365
Oussalah M, Caillet S, Saucier L, Lacroix M (2007) Inhibitory effects of selected plant essential oils on the growth of four pathogenic bacteria: E. coli O157:H7, Salmonella typhimurium, Staphylococcus aureus and Listeria monocytogenes. Food Control 18(5):414–420. https://doi.org/10.1016/j.foodcont.2005.11.009
Palazzo MC, Agius BR, Wright BS, Haber WA, Moriarity DM, Setzer WN (2009) Chemical composition and cytotoxic activities of leaf essential oils of four Lauraceae tree species from Monteverde. Costa Rica Rec Nat Prod 3(1):32–37
Perez-Lopez A, Cirio AT, Rivas-Galindo VM, Aranda RS, de Torres NW (2011) Activity against Streptococcus pneumoniae of the essential oil and δ-cadinene isolated from Schinus molle fruit. J Essent Oil Res 23(5):25–28. https://doi.org/10.1080/10412905.2011.9700477
Russo R, Corasaniti MT, Bagetta G, Morrone LA (2015) Exploitation of cytotoxicity of some essential oils for translation in cancer therapy. Evid Based Complement Alternat Med 2015:397821. https://doi.org/10.1155/2015/397821
Saikia S, Tamuli KJ, Narzary B, Banik D, Bordoloi M (2020) Chemical characterization, antimicrobial activity, and cytotoxic activity of Mikania micrantha Kunth flower essential oil from North East India. Chem Pap 74(8):2515–2528. https://doi.org/10.1007/s11696-020-01077-6
Saqib F, Ahmed MG, Janbaz KH, Dewanjee S, Jaafar HZE, Zia-Ul-Haq M (2015) Validation of ethnopharmacological uses of Murraya paniculata in disorders of diarrhea, asthma and hypertension. BMC Complement Altern Med 15:319. https://doi.org/10.1186/s12906-015-0837-7
Sarker SD, Nahar L, Kumarasamy Y (2007) Microtitre plate-based antibacterial assay incorporating resazurin as an indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods 42(4):321–324. https://doi.org/10.1016/j.ymeth.2007.01.006
Selestino Neta MC, Vittorazzi C, Guimaraes AC, Lopes Martins JD, Fronza M, Endringer DC, Scherer R (2016) Effects of beta-caryophyllene and Murraya paniculata essential oil in the murine hepatoma cells and in the bacteria and fungi 24-h time-kill curve studies. Pharm Biol 55(1):190–197. https://doi.org/10.1080/13880209.2016.1254251
Selvamani V, Zareei A, Elkashif A, Maruthamuthu MK, Chittiboyina S, Delisi DA, Li Z, Cai L, Pol V, Seleem M, Rahimi R (2020) Hierarchical micro/mesoporous copper structure with enhanced antimicrobial property via laser surface texturing. Adv Mater Interfaces 7(7):1901890. https://doi.org/10.1002/admi.201901890
Semarayani CIM, Aziz SA, Melati M (2018) Essential oil production of Murraya paniculata (L.) Jack at different harvest times. Adv Hortic Sci 32(4):471–477. https://doi.org/10.13128/ahs-21989
Sikkema J, De Bont JAM, Poolman B (1995) Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev 59(2):201–222. https://doi.org/10.1128/mr.59.2.201-222.1995
Togashi N, Shiraishi A, Nishizaka M, Matsuoka K, Endo K, Hamashima H, Inoue Y (2007) Antibacterial activity of long-chain fatty alcohols against Staphylococcus aureus. Molecules 12(2):139–148. https://doi.org/10.3390/12020139
Torey A, Vijayarathna S, Jothy SL, Gothai S, Chen Y, Latha LY, Kanwar JR, Dharmaraj S, Sasidharan S (2016) Exploration of the anticandidal mechanism of Cassia spectabilis in debilitating candidiasis. J Trad Complement Med 6(1):97–104. https://doi.org/10.1016/j.jtcme.2014.11.017
Verma N, Shukla S (2015) Impact of various factors responsible for fluctuation in plant secondary metabolites. J Appl Res Med Aromat Plants 2(4):105–113. https://doi.org/10.1016/j.jarmap.2015.09.002
WHO report on cancer: setting priorities, investing wisely and providing care for all Geneva: World Health Organization (2020) Licence: CC BY-NC-SA 3.0 IGO
WHO (2017) http://www.who.int/medicines/publications/WHO-PPL-Short_Summary_25Feb-ET_NM_WHO.pdf. Accessed 3 Nov 2020
Wiegand I, Hilpert K, Hancock REW (2008) Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc 3:163–175. https://doi.org/10.1038/nprot.2007.521
Zhang JY, Li N, Che YY, Zhang Y, Liang SX, Zhao MB, Jiang Y, Tu PF (2011) Characterization of seventy polymethoxylated flavonoids (PMFs) in the leaves of Murraya paniculata by on-line high-performance liquid chromatography coupled to photodiode array detection and electrospray tandem mass spectrometry. J Pharm Biomed Anal 56(5):950–961. https://doi.org/10.1016/j.jpba.2011.08.019
Acknowledgements
We thank the Director, CSIR-NEIST, Jorhat, Assam for providing laboratory facilities. The work was supported by CSIR-New Delhi for CSC-0130/207 and DBT, India for BT/PR25189/NER/95/1067/2017.
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This article was funded by Council of Scientific and Industrial Research, India (CSC-130/207), Department of Biotechnology, Ministry of Science and Technology (BT/PR25189/NER/95/1067/2017).
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Saikia, S., Tamuli, K.J., Narzary, B. et al. Chemical composition, antimicrobial activity and cytotoxicity of Murraya paniculata (L.) Jack leaf essential oil from Assam, India: the effect of oil on cellular morphology of micro-organisms. Arch Microbiol 204, 99 (2022). https://doi.org/10.1007/s00203-021-02665-0
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DOI: https://doi.org/10.1007/s00203-021-02665-0