Abstract
Typhoid fever is caused by the bacteria Salmonella enterica subspecies enterica serovar Typhi (S. Typhi) and remains a significant health problem in many developing countries. Lack of adequate diagnostic capabilities has contributed greatly in making typhoid fever endemic in these regions. Reliable and inexpensive diagnostic tests are needed to improve the management of this disease burden. We evaluated the ability of staA, viaB and sopE genes to detect and differentiate between the three most prevalent Salmonella spp. in Kenya (S. Typhi, S. Typhimurium and S. Enteritidis) using conventional polymerase chain reaction (PCR). The staA primers and viaB primers were found to be specific only for the different strains of S. Typhi, producing PCR products of 585 bp and 540 bp, respectively. The sopE primers was demonstrated to be specific for all Salmonella spp. producing a 465 bp PCR product with no amplification with E. coli and S. boydii bacterial strains.
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Acknowledgements
We would also like to thank the Centre for Microbiology Research at the Kenya Medical Research Institute and Kenyatta National Hospital in Nairobi, Kenya for providing access to different bacterial strains and raw samples.
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This study was funded by the National Council for Science and Technology (NACOSTI) Kenya.
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FK acquired, analyzed and interpreted the data, drafted and revised the manuscript. PG acquired the data, evaluated and interpreted antimicrobial resistance data. The concept and design of the study and manuscript revision were performed by FK, AN, GJ, PK, JK. All authors read and approved the manuscript.
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Communicated by Erko Stackebrandt.
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Kariuki, F., Getanda, P., Nyachieo, A. et al. Evaluation of the detection of staA, viaB and sopE genes in Salmonella spp. using the polymerase chain reaction (PCR). Arch Microbiol 204, 25 (2022). https://doi.org/10.1007/s00203-021-02654-3
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DOI: https://doi.org/10.1007/s00203-021-02654-3