Abstract
In this work, a strain named YPW1 was isolated from the sediments of an artificial mangrove in Yanpu harbor, China. A complete genome of YPW1 was sequenced and assembled. The 16S rRNA gene assigned strain YPW1 into genus Microbulbifer, and the maximum values of average nucleotide identity and digital DNA–DNA hybridization of ZHDP1 genome were 90.36 and 68.1, respectively, indicating that YPW1 was a potential new species in genus Microbulbifer. A total of 10 representative genomes from genus Microbulbifer were selected to compare with YPW1. The results showed that the genome of strain YPW1 possessed more carbohydrate-active enzyme genes to transform various recalcitrant polysaccharides into bioavailable monosaccharides than those of the selected genomes. Furthermore, among the selected genomes, YPW1 was the only strain with nitrate, nitrite, and nitric oxide reductases which could appoint nitrous oxide, a powerful greenhouse gas, as the end-product of its denitrification process. Therefore, strain YPW1 was a potential novel member of genus Microbulbifer with special ecological roles in the cycles of carbon and nitrogen in mangrove ecosystems.
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The genome sequence has been deposited in Genbank database under the accession no. CP055157.
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Acknowledgements
Authors sincerely thank all the members of Bonian Shui’s research group for their kindly assistance in the sampling work, and we also thank Jie Wu for the valuable inspiration for the study of polysaccharide metabolism.
Funding
This research was supported by grants from Science and Technology Program of Zhoushan (No. 2021C21009), Technology Innovation Center for Exploitation of Marine Biological Resources, MNR (No. TICMBR202102), and College Students' Innovative Entrepreneurial Training Plan Program (202010340007).
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Communicated by Kristina Beblo-Vranesevic.
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Wang, D., Wang, J., Shui, B. et al. Complete genome sequence of Microbulbifer sp. YPW1 from mangrove sediments in Yanpu harbor, China. Arch Microbiol 203, 6143–6151 (2021). https://doi.org/10.1007/s00203-021-02578-y
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DOI: https://doi.org/10.1007/s00203-021-02578-y