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Glycomyces salinus sp. nov., an actinomycete isolated from a hypersaline habitat

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Abstract

A Gram-stain-positive, aerobic, nonmotile actinobacterium, designated strain YIM 93776T, was isolated from a saline sediment sample collected from Aiding Lake in Xinjiang Uygur Autonomous Region, Northwest China. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain YIM 93776T was affiliated to the genus Glycomyces and was closely related to Glycomyces albus TRM 49136T (97.6% sequence similarity), Glycomyces lacisalsi XHU 5089T (97.0%) and Glycomyces anabasis EGI 6500139T (96.2%). The cell wall contained meso-diaminopimelic acid and the whole-cell hydrolysates sugars were galactose, mannose, arabinase, glucose and ribose. The predominant menaquinones were MK-9 (H4) and MK-10 (H4). Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two phosphatidylglyceride, two unidentified phospholipids and two unidentified polar lipids were detected in the polar lipid extracts. Major fatty acids were anteiso-C17:0, iso-C15:0, iso-C16:0, anteiso-C15:0 and anteiso C17:1 A. The draft genome sequence of strain YIM 93776T was 5.37 Mbp in size with 69.5% DNA G + C content. The dDDH and ANI values between strain YIM 93776T and related neighbours were 25.0–34.3% and 77.3–79.8%, respectively. On the basis of morphological, chemotaxonomic and phylogenetic evidence, strain YIM 93776T; therefore, represents a novel species, for which the name Glycomyces salinus sp. nov. is proposed. The type strain is YIM 93776T (= KCTC 49430T = CGMCC 4.7685T).

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Abbreviations

CA:

Czapek’s agar

PDA:

Potato dextrose agar

NA:

Nutrient agar media

ISP 3:

Oat agar

ISP 5:

Glycerol/asparagine agar

ISP 4:

Inorganic salts–starch agar

ISP2:

Yeast extract–malt extract agar

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

PGL:

Phosphatidylglyceride

UL:

Unidentified polar lipids

PI:

Phosphatidylinositol

PL:

Unidentified phospholipids

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Acknowledgements

This study was supported by the National Natural Science Foundation of China (31760003 and 31970122), the Natural Science Foundation of Yunnan Province (202001BB050040) and Major Science and Technology Projects of Yunnan Province (Digitalization, development and application of biotic resource 202002AA100007).

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Author notes

  1. Rui Li and Gang-Qiang Jiang contributed equally to this work.

Authors and Affiliations

  1. Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-Resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming, 650091, People’s Republic of China

    Rui Li & Shu-Kun Tang

  2. Urumqi Customs Technology Center, Urumqi, 830063, People’s Republic of China

    Gang-Qiang Jiang

  3. Life Science and Technology School, Lingnan Normal University, Zhanjiang, 524048, People’s Republic of China

    Yun Wang

  4. National and Local United Engineering Laboratory of Integrative Utilization Technology of Eucommia Ulmoides, College of Biology and Environmental Sciences, Jishou University, Jishou, 416000, People’s Republic of China

    Yi-Guang Chen

  5. International Joint Research Center for Karstology, School of Earth Sciences, Yunnan University, Kunming, 650091, People’s Republic of China

    En-Min Zhou

Authors
  1. Rui Li
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  2. Gang-Qiang Jiang
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  3. Yun Wang
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  4. Yi-Guang Chen
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Contributions

RL and E-MZ carried out the data analysis and wrote the manuscript. RL, YW and Y-GC performed the experiments. G-QJ provided the samples. E-MZ and S-KT supervised the project. All the authors discussed the results and contributed to the final manuscript.

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Correspondence to En-Min Zhou or Shu-Kun Tang.

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Communicated by Erko Stackebrandt.

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Li, R., Jiang, GQ., Wang, Y. et al. Glycomyces salinus sp. nov., an actinomycete isolated from a hypersaline habitat. Arch Microbiol 203, 5249–5255 (2021). https://doi.org/10.1007/s00203-021-02510-4

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  • DOI: https://doi.org/10.1007/s00203-021-02510-4

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