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Interaction of magnolia bark extracts with Staphylococcus aureus DNA and evaluation of the stability of their antibacterial activities

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Abstract

Magnolia bark is an edible traditional Chinese medicine that has antibacterial activity against Staphylococcus aureus. In the present study, interactions between S. aureus DNA and raw magnolia bark (RMB) and ginger mix-fried magnolia bark (GMB) aqueous extracts were determined via spectroscopic methods. Fluorescence spectroscopy and Stern–Volmer constants showed that S. aureus DNA quenched the fluorescence of the extracts by static quenching. UV–Vis spectroscopy and iodide quenching experiments indicated that the interactions between S. aureus DNA and the fluorescent substances might involve groove binding or electrostatic interactions. In 4′, 6-diamidino-2-phenylindole competitive assays, the fluorescence intensity at decreased as the extract amount was increased. This indicates that groove binding is responsible for the fluorescence quenching. The antibacterial activity of GMB aqueous extract treated under light, cold, heat and cycling hot–cold conditions decreased by 13.99, 9.31, 10.89 and 14.40%, respectively, whereas that of RMB aqueous extract treated under the same conditions decreased by 8.91, 14.99, 14.99 and 13.70%, respectively. The results indicate that S. aureus DNA quenches the fluorescence of GMB and RMB aqueous extracts by grooving interactions. Additionally, the antibacterial activities of GMB and RMB extracts are sensitive to light and temperature, respectively.

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Acknowledgements

This study was supported by a grant from the National Natural Science Foundation of China (No. 31401390). The authors also thank the Promotional Project for Postgraduate Research Capabilities (2017) for their financial support.

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Authors and Affiliations

  1. Beijing Key Laboratory of Plant Resources Research and Development, College of Chemistry and Materials Engineering, Beijing Technology and Business University, Beijing, China

    Shu-Mei Cui, Hai-yun Liang, Ting Li, Ke-Ke He, Yu-Mei Zheng, Meng Tang & Li-Ya Song

  2. College of Life Sciences, Fujian Normal University, Fuzhou, 350117, China

    Shu-Mei Cui & Chong-rong Ke

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  1. Shu-Mei Cui
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  2. Hai-yun Liang
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Correspondence to Li-Ya Song.

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Communicated by Erko Stackebrandt.

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Cui, SM., Liang, Hy., Li, T. et al. Interaction of magnolia bark extracts with Staphylococcus aureus DNA and evaluation of the stability of their antibacterial activities. Arch Microbiol 203, 5215–5224 (2021). https://doi.org/10.1007/s00203-021-02501-5

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  • DOI: https://doi.org/10.1007/s00203-021-02501-5

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