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Gelidibacter maritimus sp. nov., isolated from marine sediment

Abstract

A Gram-stain-negative, yellow, strictly aerobic, non-flagellated, gliding, rod-shaped bacterial strain, was isolated from costal sediment, designated as F6074T. The strain F6074T grows optimally at 30 °C, pH 7.5, and 3.0% (w/v) NaCl. Cells of strain F6074T are 0.2–0.5 µm wide and 1.0–2.0 µm long. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain F6074T belonged to the genus Gelidibacter, with the highest sequence similarity to Gelidibacter japonicus JCM 31967T (98.0%), followed by G. flavus JCM 31135T (97.7%), and similarity between strain F6074T and the type species G. algens DSM 12408T was 96.0%. Genome sequencing results revealed a genome size of 47,07,621 bp. The DNA G + C content was 37.8 mol%. The ANI and dDDH values between strain F6074T and G. japonicus JCM 31967T were 83.9 and 27.8%, the values between strain F6074T and G. algens DSM 12408T were 77.5% and 31.5%, and the values between strain F6074T and G. flavus JCM 31135T were 84.3 and 27.9%, respectively. The predominant quinone was MK-6 and the major fatty acids were iso-C15:0, iso-C15:1G, iso-C17:0 3-OH, anteiso-C15:0 and summed feature 3. The polar lipids were consisted of phosphatidylethanolamine (PE), two unidentified aminolipids (AL) and three unidentified lipids (L1, L2, L3). Based on the phenotypic, phylogenetic and chemotaxonomic data, strain F6074T was considered to represent a novel species of the genus Gelidibacter, for which the name Gelidibacter maritimus sp. nov., is proposed. The type strain is F6074T (MCCC 1H00427T = KCTC 72942T).

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Acknowledgments

The work of scanning electron microscope was supported by Physical–Chemical Materials Analytical and Testing Center of Shandong University at Weihai.

Funding

This work was supported by the National Natural Science Foundation of China (32070002, 31770002) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).

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Strain F6074T was isolated by CG and HYL. Material preparation, data collection and analysis were performed by CG, HYL, DDS and MC. The manuscript was written by CG. Project guidance and critical revision of manuscripts were performed by ZJD and MQY. All authors read and approved the final manuscript.

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Correspondence to Meng-Qi Ye or Zong-Jun Du.

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Communicated by Erko Stackebrandt.

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Gao, C., Lun, HY., Shang, DD. et al. Gelidibacter maritimus sp. nov., isolated from marine sediment. Arch Microbiol (2021). https://doi.org/10.1007/s00203-021-02478-1

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Keywords

  • Gelidibacter maritimus
  • Flavobacteriaceae
  • 16S rRNA gene
  • Polyphasic taxonomy