A Gram-stain-negative, yellow, strictly aerobic, non-flagellated, gliding, rod-shaped bacterial strain, was isolated from costal sediment, designated as F6074T. The strain F6074T grows optimally at 30 °C, pH 7.5, and 3.0% (w/v) NaCl. Cells of strain F6074T are 0.2–0.5 µm wide and 1.0–2.0 µm long. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain F6074T belonged to the genus Gelidibacter, with the highest sequence similarity to Gelidibacter japonicus JCM 31967T (98.0%), followed by G. flavus JCM 31135T (97.7%), and similarity between strain F6074T and the type species G. algens DSM 12408T was 96.0%. Genome sequencing results revealed a genome size of 47,07,621 bp. The DNA G + C content was 37.8 mol%. The ANI and dDDH values between strain F6074T and G. japonicus JCM 31967T were 83.9 and 27.8%, the values between strain F6074T and G. algens DSM 12408T were 77.5% and 31.5%, and the values between strain F6074T and G. flavus JCM 31135T were 84.3 and 27.9%, respectively. The predominant quinone was MK-6 and the major fatty acids were iso-C15:0, iso-C15:1G, iso-C17:0 3-OH, anteiso-C15:0 and summed feature 3. The polar lipids were consisted of phosphatidylethanolamine (PE), two unidentified aminolipids (AL) and three unidentified lipids (L1, L2, L3). Based on the phenotypic, phylogenetic and chemotaxonomic data, strain F6074T was considered to represent a novel species of the genus Gelidibacter, for which the name Gelidibacter maritimus sp. nov., is proposed. The type strain is F6074T (MCCC 1H00427T = KCTC 72942T).
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Biebl H, Pukall R, Lünsdorf H, Schulz S, Allgaier M, Tindall BJ, Wagner-Dobler I (2007) Description of Labrenzia alexandrii gen. nov., sp. nov., a novel alphaproteobacterium containing bacteriochlorophyll a, and a proposal reclassifcation of Stappia aggregata as Labrenzia aggregata comb. nov., and of Stappia alba as Labrenzia alba comb. nov. Int J Syst Evol Microb 57:1095–1107
Blin K, Shaw S, Steinke K (2019) antiSMASH 5.0: updates to the secondary metabolite genome mining pipeline. Nucleic Acids Res 47:W81–W87
Bowman JP (2000) Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassifcation of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 50:1861–1868
Bowman JP, Nichols DS (2005) Novel members of the family Flavobacteriaceae from Antarctic maritime habitats including Subsaximicrobium wynnwilliamsii gen. nov., sp. nov., Subsaximicrobium saxinquilinus sp. nov., Subsaxibacter broadyi gen. nov., sp. nov., Lacinutrix copepodicola gen. nov., sp. nov., and novel species of the genera Bizionia, Gelidibacter and Gillisia. Int J Syst Evol Microbiol 55(4):1471–1486
Bowman JP, McCammon SA, Brown JL, Nichols PD, McMeekin TA (1997) Psychroserpens burtonensis gen. nov., sp. nov., and Gelidibacter algens gen. nov., sp. nov., psychrophilic bacteria isolated from Antarctic Lacustrine and Sea Ice Habitats. Int J Syst Evol Microbiol 47(3):670–677
Collins MD, Pirouz T, Goodfellow M, Minnikin DE (1977) Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100:221–230
Cowan ST, Steel KJ (1974) Bacterial characters and characterization, 2nd edn. Cambridge University Press, Cambridge
Doi H, Osawa I (2019) Description of Gelidibacter japonicus sp. nov., isolated from the Inland Sea (Setonaikai) in Japan. Arch Microbiol 201(8):1019–1024
Dong XZ, Cai MY (2001) Determination of biochemical characteristics. Manual for the systematic identifcation of general bacteria, 14th edn. Science Press, Beijing, pp 370–398
Du ZJ, Wang Y, Dunlap C, Rooney AP, Chen GJ (2014) Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov. Int J Syst Evol Microbiol 64:1690–1696
Goris J, Konstantinidis KT, Klappenbach JA (2007) DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. Int J Syst Evol Microbiol 57(1):81–91
Kim HS, Bang JJ, Lee SS (2017) Gelidibacter flavus sp. nov., isolated from activated sludge of seawater treatment system. Curr Microbiol 74(11):1247–1252
Kroppenstedt RM (1982) Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chrom 5:2359–2367
Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874
Liu QQ, Wang Y, Li J, Du ZJ, Chen GJ (2014) Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans. Int J Syst Evol Microbiol 64(7):2204–2209
Macián MC, Pujalte MJ, Márquez MC, Ludwig W, Ventosa A, Garay E, Schleifer KH (2002) Gelidibacter mesophilus sp. nov., a novel marine bacterium in the family Flavobacteriaceae. Int J Syst Evol Microbiol 52(4):1325–1329
Meier-Kolthoff J, Auch A, Klenk HP, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14:60
Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett JH (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Method 2:233–241
Shang D-D, Lun H-Y, Zhu K-L, Chen G-J, Du Z-J (2021) Tenacibaculum pelagium sp. nov., isolated from marine sediment. Arch Microbiol 203(5):2229–2236. https://doi.org/10.1007/S00203-021-02208-7
Smibert RM, Krieg NR (1994) Phenotypic characterization. In: Gerhardt P, Murray RGE, Wood WA, Krieg NR (eds) Methods for general, molecular bacteriology. American Society for Microbiology, Washington, pp 607–654
Strandwitz P, Kim KH, Terekhova D, Liu JK, Sharma A, Levering J, McDonald D, Dietrich D, Ramadhar TR, Lekbua A, Mroue N, Liston C, Stewart EJ, Dubin MJ, Zengler K, Knight R, Gilbert JA, Clardy J, Lewis K (2019) GABA-modulating bacteria of the human gut microbiota. Nat Microbiol 4(3):396–403
Dan-Dan S, Lun H-Y, Zhu K-L, Chen G-J, Zong-Jun Du (2021) Tenacibaculum pelagium sp. nov., isolated from marine sediment. Arch Microbiol. https://doi.org/10.1007/S00203-021-02208-7
Tindall BJ, Sikorski J, Smibert RA, Krieg NR (2007) Phenotypic characterization and the principles of comparative systematics. In: Reddy CA, Beveridge TJ, Breznak JA, Marzluf GA, Schmidt TM, Snyder LR et al (eds) Methods for general and molecular bacteriology, 3rd edn. American Society for Microbiology, Washington, pp 330–393
Yarza P, Yilmaz P, Pruesse E, Glöckner FO, Ludwig W, Schleifer KH, Whitman WB, Euzéby J, Amann R, Rosselló-Móra R (2014) Uniting the classification of cultured and uncultured bacteria and archaea using 16S rRNA gene sequences. Nat Rev Microbiol 12:635–645
Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J (2017) Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 67:1613–1617
Zhang D-C, Margesin R (2015) Gelidibacter sediminis sp. nov., isolated from a sediment sample of the Yellow Sea. Int J Syst Evol Microbiol. 65(Pt_7):2304-2309. https://doi.org/10.1099/ijs.0.000256
The work of scanning electron microscope was supported by Physical–Chemical Materials Analytical and Testing Center of Shandong University at Weihai.
This work was supported by the National Natural Science Foundation of China (32070002, 31770002) and National Science and Technology Fundamental Resources Investigation Program of China (2019FY100700).
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Communicated by Erko Stackebrandt.
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Gao, C., Lun, HY., Shang, DD. et al. Gelidibacter maritimus sp. nov., isolated from marine sediment. Arch Microbiol (2021). https://doi.org/10.1007/s00203-021-02478-1
- Gelidibacter maritimus
- 16S rRNA gene
- Polyphasic taxonomy