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Devosia sediminis sp. nov., isolated from subterranean sediment

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Abstract

A novel Gram-negative, cream-colored, rod-shaped, aerobic, non-motile bacterium, designated MSA67T, was isolated from a subterranean sediment sample of the Mohe Basin in Northeast China. Strain MSA67T was detected to grow at 4–40 °C (optimum 28–30 °C), pH 5.0–10.0 (optimum, pH 7.0) and in 0.0–8.0% (w/v) NaCl (optimum 2.0–3.0%). Phylogenetic analysis based on 16S rRNA gene sequence revealed that strain MSA67T was a member of the genus Devosia, with the highest similarity with D. riboflavina IFO13584T (98.0%) and D. chinhatensis IPL18T (97.0%). The major cellular fatty acids are C16:0, C18:1ω7c 11-methyl and C18:1ω6c and/or C18:1ω7c. The major polar lipids are diphosphatidylglycerol, phosphatidylglycerol, glycolipids and three unidentified phospholipids. The major respiratory quinone is ubiquinone 10 (Q-10). The genomic size of strain MSA67T is 4.1 MB and DNA G + C content is 63.6%. Based on genotypic, phenotypic and phylogenetic results, strain MSA67T is concluded to represent a novel species of the genus Devosia, for which the name Devosia sediminis sp. nov. is proposed. The type strain is MSA67T (= CGMCC 1.18467T = KCTC 82192T).

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Abbreviations

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

COG:

Cluster of orthologous groups of proteins

RNA:

Ribonucleic acid

tRNA:

Transfer ribonucleic acid

sRNA:

Small ribonucleic acid

LINE:

Long-interspersed nuclear elements

SINE:

Short interspersed nuclear elements

RC:

Rolling circle

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

GL:

Glycolipids

L:

Unidentified lipids

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Acknowledgements

We thank Rengang Zhang for his help with sample collection and strain isolation.

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Authors and Affiliations

  1. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Fu Ma, Zhi-Da Zi, Wei Li & Jie Lv

  2. Shijiazhuang People’s Medical College, Shijiazhuang, 050091, Hebei, China

    Zhao-Xuan Wang

  3. Faculty of Health and Environmental Sciences, Auckland University of Technology, Auckland, 1142, New Zealand

    Jun Lu

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  1. Fu Ma
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  2. Zhi-Da Zi
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  4. Zhao-Xuan Wang
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Correspondence to Jie Lv.

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This study does not describe any experimental work related to human.

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Communicated by Erko Stackebrandt.

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The GenBank/EMBL/DDBJ accession numbers for the genome and 16S rRNA gene sequence of MSA67T are JAEKMH000000000 and MN006580.

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Ma, F., Zi, ZD., Li, W. et al. Devosia sediminis sp. nov., isolated from subterranean sediment. Arch Microbiol 203, 4517–4523 (2021). https://doi.org/10.1007/s00203-021-02448-7

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