Abstract
A novel F-specific RNA bacteriophage (FRNAPH) YM1, affiliating to genogroup I (GI) of Levivirus, is isolated for the first time from human stool samples using double-layer agar plates with the Escherichia coli ATCC700891 as the host. The complete genomic sequence of YM1 is 3551 nt in length, obtained through next-generation sequencing, and contains four genes encoding for maturation protein, coat protein, lysis protein, and RNA-dependent RNA polymerase (RdRp). The genomic sequence of YM1 shares the highest similarity of 95.3% with that of a GI FRNAPH DL16 isolated from surface water of Great Bay. The YM1 possesses a non-enveloped, icosahedral virion of 23 ± 0.45 nm in diameter. One-step growth curve analysis shows that the burst time of YM1 is 30 min post-infection (p.i.) with the average burst size of 264 PFU/cell. The YM1 lyses only E. coli strains tested, revealing high host specificity. This newly discovered phage may serve as a candidate for viral indicator to monitor human enteric virus, especially norovirus, contamination in the environments.
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Acknowledgements
We are grateful to Shijiazhuang Center for Disease Control and Prevention for providing the strain ATCC700891, and to Dr. Song Cong from Donghua University for providing the strain ATCC15597.
Funding
This study was funded by the National key R&D Program of China (2017YFC1600703) and the National Natural Science Foundation of China (31601570, 41876195).
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Yang, M., Yu, Y. & Wang, Y. Characterization of a novel group I F-specific RNA bacteriophage isolated from human stool. Arch Microbiol 203, 4065–4072 (2021). https://doi.org/10.1007/s00203-021-02402-7
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DOI: https://doi.org/10.1007/s00203-021-02402-7