Abstract
The need for more effective drugs for the treatment of infectious diseases as well as for general applications including wound healing and burn surgery, has guided efforts for the discovery of new compounds of medical interest. Microorganisms found in textile industrial waste have the ability to produce a variety of enzymes and/or secondary metabolites including molecules of pharmaceutical interest. The present work investigated the biotechnological potential of filamentous fungi isolated from textile industry wastewater for the production of collagenase and antimicrobial metabolites. From 28 isolates assayed, Sarocladium sp. ITF33 showed specific collagenolytic activity with values of 7.62 and 9.04 U mg−1 for the intracellular and extracellular fractions, respectively. The isolate Penicillium sp. ITF28 showed the best antimicrobial activity, reaching MIC ranging from 1.0 to 0.0625 mg mL−1 against five pathogenic bacteria. Molecular analyzes suggest that the isolate Sarocladium sp. ITF 33 can be considered a species not yet described. The results of the present work encourage studies of characterization and purification of the enzymes and secondary metabolites produced by the isolates found aiming future applications in the medical and pharmaceutical fields.
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Acknowledgements
SPFB would like to thank the Social Demand Program (Master's Scholarship) – UNILA. The authors would like to thank Mr. Fábio Muller for authorizing the sample collection from Textile Industry.
Funding
This work was supported by the Institutional Program to Support Research Groups EDITAL PRPPG Nº 110/2018 (UNILA), Program for Researcher Integration PAIP EDITAL PRPPG Nº 80/2019 (UNILA) and CONICET (National Council for Scientific and Technical Research) (PIP 112-20130100126-CO).
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MEP and MRZP conceived and designed research. SPFB, MAG and VPS conducted experiments. VMO and MEP contributed new reagents or analytical tools. MEP and MRZP analyzed data. JRO, MEP and MRZP wrote the manuscript. All authors read and approved the manuscript.
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Bernal, S.P.F., Gritti, M.A., dos Santos, V.P. et al. Pharmaceutical biotechnological potential of filamentous fungi isolated from textile industry. Arch Microbiol 203, 3933–3944 (2021). https://doi.org/10.1007/s00203-021-02379-3
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DOI: https://doi.org/10.1007/s00203-021-02379-3