Abstract
High concentrations of heavy metals in mine soil disturb the interactions between legumes and microorganisms leading to select strains adapted to these specific conditions. In this work, we analyzed the diversity of fifty strains isolated from Trifolium sp. nodules growing on Pb–Zn mine soil, in the Northeastern of Algeria and highlighted their potential symbiotic traits. The phylogeny of the 16S rRNA gene sequences revealed a high bacterial diversity with a predominance of non-rhizobial endophytes. The identified isolates belong to the thirteen following genera Cupriavidus, Pseudomonas, Bacillus, Acinetobacter, Enterobacter, Roseomonas, Paracoccus, Frondihabitans, Microbacterium, Kocuria, Providencia, Micrococcus and Staphylococcus. Regarding rhizobial strains, only isolates affiliated to Rhizobium genus were obtained. The symbiotic gene nodC and the nitrogen fixation gene nifH present showed that Rhizobium isolates belonged to the symbiovar trifolii. In addition to bacterial, one yeast strain was isolated and identified as Rhodotorula mucilaginosa by sequencing the internal transcribed spacer (ITS) region.
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The data sets used and/or analysed during the current study are available from the corresponding author (Sarah Rahal) on reasonable request.
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Acknowledgements
We are grateful to Benjamin Gourion for hosting Sarah Rahal in Laboratory of Plant-Microbe Interactions (LIPM), for the help in the experiments and his extremely useful advice throughout this research. The authors would like to thank also Laurent Sauviac, Claire Benezech and Bryan Ruiz for their help and advice.
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This work was supported by the Ministry of Higher Education and Scientific Research of Algeria. The funding bodies had no role in the design of the study and collection, analysis, and interpretation of data, or in writing the manuscript.
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SR performed the experiments. SR and DC wrote the paper.
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Rahal, S., Chekireb, D. Diversity of rhizobia and non-rhizobia endophytes isolated from root nodules of Trifolium sp. growing in lead and zinc mine site Guelma, Algeria. Arch Microbiol 203, 3839–3849 (2021). https://doi.org/10.1007/s00203-021-02362-y
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DOI: https://doi.org/10.1007/s00203-021-02362-y