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Draft genome sequencing of Sporolactobacillus terrae SBT-1, an efficient bacterium to ferment concentrated sugar to d-lactic acid

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Abstract

Recently, the industrial-scale development of microbial d-lactic acid production has been discussed. In this study, the efficiency of the new isolate Sporolactobacillus terrae SBT-1 for producing d-lactic acid under challenge conditions was investigated. The isolate SBT-1 exhibited superior activity in fermenting a very high glucose or sucrose concentration to d-lactic acid compared to the other S. terrae isolates previously reported in the literature; therefore, SBT-1 could overcome the limitations of effective lactic acid production. In batch cultivation using 360 g/L glucose, SBT-1 produced 290.30 g/L d-lactate with a sufficiently high glucose conversion yield of 86%, volumetric productivity of 3.02 g/L h, and optical purity of 96.80% enantiomer excess. SBT-1 could also effectively utilize 440 g/L sucrose as a sole carbon source to produce 276.50 g/L lactic acid with a conversion yield of 90%, a production rate of 2.88 g/L h, and an optical purity of 98%. d-Lactic acid fermentation by two other related producers, S. inulinus NRIC1133T and S. terrae NRIC0357T, was compared with fermentation by isolate SBT-1. The experimental data revealed that SBT-1 possessed the ability to ferment relatively high glucose or sucrose concentrations to d-lactic acid without obvious catabolite repression and byproduct formation compared to the two reference strains. In draft genome sequencing of S. terrae SBT-1, the results provided here can promote further study to overcome the current limitations for the industrial-scale production of d-lactic acid.

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Acknowledgements

We thank the Department of Molecular Microbiology, Faculty of Life Science, and NODAI Genome Research Center at Tokyo University of Agriculture (Japan) for supporting and facilitating the whole-genome sequence analysis.

Funding

This study was supported by the Biorefinery Cluster, Chulalongkorn University, Research Chair Grant provided by the National Science and Technology Development Agency (NSTDA), and Thailand Research Fund under Grant No. RTA6280014. Dr. Thitiprasert was the recipient of the Chulalongkorn University Office of International Affairs Scholarship Program for Short-term Research (Thailand).

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Authors and Affiliations

  1. Research Unit in Bioconversion/Bioseparation for Value-Added Chemical Production, Institute of Biotechnology and Genetic Engineering, Chulalongkorn University, Phayathai Road, Wangmai, Pathumwan, Bangkok, 10330, Thailand

    Sitanan Thitiprasert, Jirabhorn Piluk, Vasana Tolieng & Nuttha Thongchul

  2. Department of Molecular Microbiology, Faculty of Life Science, Tokyo University of Agriculture, Sakuragaoka, Tokyo, 156-8502, Japan

    Naoto Tanaka, Yuh Shiwa & Nobuyuki Fujita

  3. Research Unit in Bioconversion/Bioseparation for Value-Added Chemical Production, Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Phayathai Road, Wangmai, Pathumwan, Bangkok, 10330, Thailand

    Somboon Tanasupawat

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  1. Sitanan Thitiprasert
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Thitiprasert, S., Piluk, J., Tolieng, V. et al. Draft genome sequencing of Sporolactobacillus terrae SBT-1, an efficient bacterium to ferment concentrated sugar to d-lactic acid. Arch Microbiol 203, 3577–3590 (2021). https://doi.org/10.1007/s00203-021-02352-0

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