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Comparative genomic and secretomic characterisation of endophytic Bacillus velezensis LC1 producing bioethanol from bamboo lignocellulose

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Abstract

Bacillus is an excellent organic matter degrader, and it has exhibited various abilities required for lignocellulose degradation. Several B. velezensis strains encode lignocellulosases, however their ability to efficiently transform biomass has not been appreciated. In the present study, through the comparative genomic analysis of the whole genome sequences of 21 B. velezensis strains, CAZyome related to lignocellulose degradation was identified and their similarities and differences were compared. Subsequently, the secretome of B. velezensis LC1 by liquid chromatography-tandem mass spectrometry (LC–MS/MS) were identified and confirmed that a considerable number of proteins were involved in lignocellulose degradation. Moreover, after 6-day treatment, the degradation efficiency of the B. velezensis LC1 toward cellulose, hemicellulose and lignin were 59.90%, 75.44% and 23.41%, respectively, the hydrolysate was subjected to ethanol fermentation with Saccharomyces cerevisiae and Escherichia coli KO11, yielded 10.44 g/L ethanol after 96 h. These results indicate that B. velezensis LC1 has the ability to effectively degrade bamboo lignocellulose and has the potential to be used in bioethanol production.

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Availability of data and material

The sequence reads from this article have been deposited at the NCBI Sequence Read Archive under the accession PRJNA574012. The assembly data set supporting the results of this article has been deposited at GenBank under the accession CP044349. The version described in this paper is CP044349.

Abbreviations

B. velezensis :

Bacillus velezensis

GH:

Glycoside Hydrolase

GT:

Glycosyltransferase

CE:

Carbohydrate esterase

CBM:

Carbohydrate binding domain

PL:

Polysaccharide lyase;

AA:

Auxiliary Activity

CAZyme:

Carbohydrate-active enzyme

ABTS:

[2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)]

BP:

Bamboo powder

YPD:

Yeast Extract Peptone Dextrose Medium

DNS:

3,5-Dinitrosalicylic acid

CDS:

Sequence coding for amino acids in protein

NCBI:

National Centre for Biotechnology Information

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Acknowledgements

The data were analyzed on the free online platform of Majorbio Cloud Platform (www.majorbio.com).

Funding

This work was supported by Sichuan science and technology program (2019YFG0139) and Scientific Research Foundation of Leshan Normal University (XJR19001).

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Author notes

  1. Hao Tang, Li Zheng and Yuanqiu Li contributed equally to this work.

Authors and Affiliations

  1. College of Life Science, Central District, Leshan Normal University, No. 778, Riverside Road, Leshan, 614000, China

    Hao Tang, Li Zheng, Yuanqiu Li, Lu Lei, Xiaowen Yang & Chaobing Luo

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  1. Hao Tang
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  2. Li Zheng
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  3. Yuanqiu Li
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  4. Lu Lei
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Contributions

CBL designed and performed the experiments; HT, LZ and YQL wrote the manuscript; LZ, LL, YQL, XWY and HT analyzed the data. All authors read and approved the final manuscript.

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Correspondence to Chaobing Luo.

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This study does not include any experimental procedure performed on humans or animals.

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Communicated by Erko Stackebrandt.

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Tang, H., Zheng, L., Li, Y. et al. Comparative genomic and secretomic characterisation of endophytic Bacillus velezensis LC1 producing bioethanol from bamboo lignocellulose. Arch Microbiol 203, 3089–3099 (2021). https://doi.org/10.1007/s00203-021-02306-6

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  • DOI: https://doi.org/10.1007/s00203-021-02306-6

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