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Noviherbaspirillum pedocola sp. nov., isolated from oil-contaminated experimental soil

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Abstract

An orange-coloured, rod-shaped, and aerobic bacterial strain DKR-6 T was isolated from oil-contaminated experimental soil. The strain was Gram-stain-negative, catalase and oxidase positive, and grew at temperature 10–42 °C, at pH 5.5–9.5, and at 0–3.0% (w/v) NaCl concentration. The phylogenetic analysis and 16S rRNA gene sequence analysis suggested that the strain DKR-6 T was affiliated to the genus Noviherbaspirillum, with the closest species being Noviherbaspirillum massiliense JC206T (96.3% sequence similarity). The chemotaxonomic profiles revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylcholine as the principal polar lipids; C16:0, C17:0 cyclo, summed feature 3 (C16:1ω7c and/or C16: 1ω6c), and summed feature 8 (C18:1ω7c/or C18:1ω6c) as the main fatty acids; and Q-8 as a sole ubiquinone. The DNA G + C content was 61.6%. The polyphasic taxonomic features illustrated in this study clearly implied that strain DKR-6 T represents a novel species in the genus Noviherbaspirillum, for which the name Noviherbaspirillum pedocola sp. nov. is proposed with the type strain DKR-6 T (= KACC 22074 T = NBRC 114727 T).

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Abbreviations

KACC:

Korean agricultural culture collection

NBRC:

NITE biological resource center

DSM:

Deutsche Sammlung von Mikroorganismen

ANI:

Average nucleotide identity

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Acknowledgements

This work was supported by Subsurface Environment Management Projects funded by the Korea Environmental Industry and Technology Institute, South Korea (Project Number: 2020002440005).

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Authors and Affiliations

  1. Department of Environmental Engineering, College of Science and Technology, Korea University Sejong Campus, 2511 Sejong-ro, Sejong City, 30019, Republic of Korea

    Dhiraj Kumar Chaudhary & Yongseok Hong

  2. Department of Microbiology, School of Medicine, Kyungpook National University, Daegu, 41944, Republic of Korea

    Ram Hari Dahal

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  1. Dhiraj Kumar Chaudhary
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  2. Ram Hari Dahal
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  3. Yongseok Hong
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Correspondence to Yongseok Hong.

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The authors declare that there are no conflicts of interest regarding the publication of this manuscript.

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This study does not describe any experimental work related to human.

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Communicated by Erko Stackebrandt.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA sequence of strain DKR-6 T is MT776695. The whole genome shotgun sequence of strain DKR-6 T has been deposited at DDBJ/ENA/GenBank under the accession JAEPBG000000000. The version described in this paper is version JAEPBG010000000.

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Chaudhary, D.K., Dahal, R.H. & Hong, Y. Noviherbaspirillum pedocola sp. nov., isolated from oil-contaminated experimental soil. Arch Microbiol 203, 3071–3076 (2021). https://doi.org/10.1007/s00203-021-02295-6

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  • DOI: https://doi.org/10.1007/s00203-021-02295-6

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