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Transcriptome analysis of Cyclocarya paliurus flavonoids regulation of differently expressed genes in Enterococcus faecalis under low pH stress

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Abstract

Enterococcus faecalis (E. faecalis) is an indigenous intestinal bacterium and has potential to be applied as probiotic supplement. Low pH is one of the main stresses that E. faecalis has to deal with to colonize in the gastrointestinal tract. Previous study indicated low concentration of flavonoids may enhance the tolerance of probiotic to environmental stress. In the present research, transcriptome analysis was employed to investigate the influence of Cyclocarya paliurus flavonoids (CPF) on E. faecalis exposed to low pH environment. The results revealed that under the stress of low pH, genes related to cell wall and membrane, transmembrane transport, metabolism process, energy production, and conversion stress proteins were significantly differentially expressed. And certain undesired changes of which (such as genes for MFS transporter were downregulated) could be partially mitigated by CPF intervention, indicating their capacity to improve the low pH tolerance of E. faecalis. Results from this study deepened our understanding of the beneficial role of CPF on the probiotic in the gastrointestinal environment.

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Acknowledgements

This work was supported by the Fundamental Research Funds for the Central Universities (2020SKTY01), the National Natural Science Foundation of China (31650006), "Yueqi Young Scholars" Funding Program of China University of Mining and Technology (Beijing), and the Key Research and Development Project of Zhejiang Province (2018C02047).

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Authors and Affiliations

  1. Department of Physical Education, China University of Mining and Technology, Beijing, 100083, People’s Republic of China

    Li Zhang

  2. Department of Food Science and Engineering, Ningbo University, Ningbo, 315211, People’s Republic of China

    Dan Song & Zufang Wu

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  1. Li Zhang
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  2. Dan Song
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  3. Zufang Wu
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Correspondence to Zufang Wu.

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Communicated by Erko Stackebrandt.

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Supplementary file 1. Figure S1.

The electron micrographs of E. faecalis 131-2 from SEM. A (control group), B (acidified group) and C (acidified-CPF group).

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Zhang, L., Song, D. & Wu, Z. Transcriptome analysis of Cyclocarya paliurus flavonoids regulation of differently expressed genes in Enterococcus faecalis under low pH stress. Arch Microbiol 203, 2147–2155 (2021). https://doi.org/10.1007/s00203-021-02215-8

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  • DOI: https://doi.org/10.1007/s00203-021-02215-8

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