Two bacterial strains, designated REN4T and REN4-1, were isolated from daqu sample collected from baijiu factory located in Shanxi, China. The two strains shared highly similar 16S rRNA gene sequences (99.67% identities) and formed a monophyletic clade within the Brevibacterium 16S rRNA gene tree, showing 97.56–97.85% 16S rRNA gene sequence identities with type strains Brevibacterium permense VKM Ac-2280 T, Brevibacterium sediminis FXJ8.269 T, Brevibacterium oceani BBH7T and Brevibacterium epidermidis NCIMB 702286 T. They contained MK-8(H2) as the most predominant menaquinone, antesio-C15:0, antesio-C17:0, Iso-C16:0 and Iso-C17:0 as the major cellular fatty acids, DPG (diphosphatidylglycerol), PG (phosphatidylglycerol), PGL (phosphatidylglycerollipids), and PL (phospholipids) as the main polar lipids. The genomic DNA G + C content of strains REN4 and REN4-1 were 64.35, 65.82 mol%. Moreover, the low DNA-DNA relatedness values, physiological and biochemical characteristics, and taxonomic analysis allowed the differentiation of strains REN4T and REN4-1 from the other recognized species of the genus Brevibacterium. Therefore, strain REN4T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium renqingii sp. nov. is proposed, with the type strain REN4T (= JCM 33953 T = KCTC 49366 T).
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Average nucleotide identity
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We really appreciate Dr. Ren Qing’s help on the provision of baijiu sample and some technical support.
This work supported by the National Key Research and Development Program of China (2018YFC1603606)
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Communicated by Erko Stackebrandt.
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Supplementary file 3 Fig. S3. Two-dimensional thin-layer chromatography of the total polar lipids of strain REN4T. The thin-layer chromatography plate was stained for total polar lipids with 5% ethanolic molybdophosphoric acid. DPG, diphosphatidylglycerol; PG, phosphatidylglycerol; PGL, phosphatidylglycerollipids; PL, Phospholipids. L, spraying with molybdatophosphoric acid reagent. (TIF 2217 KB)
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Yan, Y., Xing, X., Sun, Z. et al. Brevibacterium renqingii sp. nov., isolated from the Daqu of Baijiu. Arch Microbiol (2021). https://doi.org/10.1007/s00203-021-02212-x
- Brevibacterium renqingii
- Polyphasic taxonomy
- 16S rRNA