Brevibacterium renqingii sp. nov., isolated from the Daqu of Baijiu

Abstract

Two bacterial strains, designated REN4T and REN4-1, were isolated from daqu sample collected from baijiu factory located in Shanxi, China. The two strains shared highly similar 16S rRNA gene sequences (99.67% identities) and formed a monophyletic clade within the Brevibacterium 16S rRNA gene tree, showing 97.56–97.85% 16S rRNA gene sequence identities with type strains Brevibacterium permense VKM Ac-2280 T, Brevibacterium sediminis FXJ8.269 T, Brevibacterium oceani BBH7T and Brevibacterium epidermidis NCIMB 702286 T. They contained MK-8(H2) as the most predominant menaquinone, antesio-C15:0, antesio-C17:0, Iso-C16:0 and Iso-C17:0 as the major cellular fatty acids, DPG (diphosphatidylglycerol), PG (phosphatidylglycerol), PGL (phosphatidylglycerollipids), and PL (phospholipids) as the main polar lipids. The genomic DNA G + C content of strains REN4 and REN4-1 were 64.35, 65.82 mol%. Moreover, the low DNA-DNA relatedness values, physiological and biochemical characteristics, and taxonomic analysis allowed the differentiation of strains REN4T and REN4-1 from the other recognized species of the genus Brevibacterium. Therefore, strain REN4T represents a novel species of the genus Brevibacterium, for which the name Brevibacterium renqingii sp. nov. is proposed, with the type strain REN4T (= JCM 33953 T = KCTC 49366 T).

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Fig. 1

Abbreviations

ANI:

Average nucleotide identity

DPG:

Diphosphatidylglycerol

PG:

Phosphatidylglycerol

PGL:

Phosphatidylglycerollipids

PL:

Phospholipids

DDH:

DNA-DNA hybridization

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Acknowledgement

We really appreciate Dr. Ren Qing’s help on the provision of baijiu sample and some technical support.

Funding

This work supported by the National Key Research and Development Program of China (2018YFC1603606)

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J.L.X.; methodology, X.X. and Y.Y.; performed the experiments and wrote the manuscript, Z.B.S., J.L. and S.Y.H; experiment.

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Correspondence to Jialiang Xu.

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Communicated by Erko Stackebrandt.

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Yan, Y., Xing, X., Sun, Z. et al. Brevibacterium renqingii sp. nov., isolated from the Daqu of Baijiu. Arch Microbiol (2021). https://doi.org/10.1007/s00203-021-02212-x

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Keywords

  • Brevibacterium renqingii
  • Polyphasic taxonomy
  • 16S rRNA
  • Baijiu